The development of sustainable and renewable biofuels is attracting growing interest. It is vital to develop robust microbial strains for biocatalysts that are able to function under multiple stress conditions. This Microbiology Monograph provides an overview of methods for studying microbial stress tolerance for biofuels applications using a systems biology approach.

Topics covered range from mechanisms to methodology for yeast and bacteria, including the genomics of yeast tolerance and detoxification; genetics and regulation of glycogen and trehalose metabolism; programmed cell death; high gravity fermentations; ethanol tolerance; improving biomass sugar utilization by engineered Saccharomyces; the genomics on tolerance of Zymomonas mobilis; microbial solvent tolerance; control of stress tolerance in bacterial host organisms; metabolomics for ethanologenic yeast; automated proteomics work cell systems for strain improvement; and unification of gene expression data for comparable analyses under stress conditions.

Genomics of yeast tolerance and in situ detoxification

Genetics and regulation of glycogen and trehalose metabolism in Saccharomyces cerevisiae
Molecular mechanisms of programmed cell death induced by acetic acid in Saccharomyces cerevisiae
Molecular mechanisms of ethanol tolerance in Saccharomyces cerevisiae
High gravity ethanol fermentations and yeast tolerance
Improving biomass sugar utilization by engineered Saccharomyces cerevisiae
Genomics on pretreatment inhibitor tolerance of Zymomonas mobilis
Mechanisms and applications of microbial solvent tolerance
Control of stress tolerance in bacterial host organisms for bioproduction of fuels
Metabolomics for ethanologenic yeast
Automated systems of plasmid-based functional proteomics to improve microbes for biofuel production
Unification of gene expression data for comparable analyses under stress conditions.