A Practical Guide to Protein Engineering
A Practical Guide to Protein Engineering
This textbook introduces readers in an accessible and engaging way to the nuts and bolts of protein expression and engineering. Various case studies illustrate each step from the early sequence searches in online databases over plasmid design and molecular cloning techniques to protein purification and characterization. Furthermore, readers are provided with practical tips to successfully pursue a career as a protein engineer. With protein engineering being a fundamental technique in almost all molecular biology labs, the book targets advanced undergraduates and graduate students working in molecular biology, biotechnology and related scientific fields.
Literature Search
Sequence AnalysisStructural Analysis
Protein Expression Hosts and Expression Plasmids
Gene Cloning
Protein Expression
Assay
Gene Mutagenesis
High-Throughput Screening (HTS)
Protein Variants Analysis and Characterization
Continuing from Protein Variants
Employability.
ISBN | 978-3-030-56897-9 |
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Artikelnummer | 9783030568979 |
Medientyp | Buch |
Auflage | 1st ed. 2020 |
Copyrightjahr | 2020 |
Verlag | Springer, Berlin |
Umfang | XV, 202 Seiten |
Abbildungen | XV, 202 p. 91 illus., 68 illus. in color. |
Sprache | Englisch |