"Advanced Techniques in Soil Microbiology" presents a wide range of biotechnological methods for application in soil microbiology analysis. These include all essential methods involving molecular biology, immunology, microbiology, and structural biology, such as transcriptome analysis, RNAi technology, molecular matchmaking, RAPD, T-RFLP and FT/MS.

The techniques and procedures have been selected with the aim of offering practical guides for immediate use in the laboratory. The systems investigated range from individual molecules and cells to entire eukaryotic organisms, with a focus on bacteria, fungi, mycorrhiza, and higher plants. This volume of state-of-the-art, practice oriented methods will be of great use both to the first-timer and to the experienced scientist.

Detection and Diversity of Fungi from Environmental Samples: Traditional Versus Molecular Approaches

Functional Genomic Approaches for Mycorrhizal Research
Automated Fluoroscence Sequencing and Troubleshooting
mRNA Quantitation Using Real Time PCR
Laboratory Practice for the Production of Polyclonal and Monoclonal Antibodies
Modern Techniques for Analyzing Immunological Responses
Transcriptome Analysis
RNAi Technology: a Tool for Functional Validation of Novel Genes
Molecular Matchmaking: Techniques for Biomolecular Interactions
Environmental Proteomics: Extraction and Identification of Protein in Soil
DGGE and RISA Protocols for Microbial Community Analysis in Soil
Soil Microbial Community Structure and Function Assessed by FAME, PLFA and DGGE - Advantages and Limitations
Measurement of Microbial Biomass and Activity in Soil
Immuno-Technology for the Localization of Acid Phosphatase Using Native Gel Bands in Piriformospora indica and Other Soil Microorganisms
Use of Short Oligonucleotide Primers in Random Amplified Polymorphic DNA Techniques for Species Identification
Co-Cultivation with Sebacinales
Quantitative Histochemistry: a Forgotten Tool with New Applications
Ion Cyclotron Resonance Fourier Transform Mass Spectrometry for Non-Targeted Metabolomics of Molecular Interactions in the Rhizosphere
Application of Terminal-Restriction Fragment Length Polymorphism for MolecularAnalysis of Soil Bacterial Communities
Molecular Symbiotic Analysis Between Arabiopsis thaliana and Piriformospora indica
Biophysical Phenomics Reveals Functional Building Blocks of Plants Systems Biology: a Case Study for the Evaluation of the Impact of Mycorrhization with Piriformospora indica
Analysis of the Plant Protective Potential of the RootEndophytic Fungus Piriformospora indica in Cereals
Members of Sebacinales Confer Resistance Against Heavy Metal Stress in Plants
Screening of Plant Growth-Promoting Rhizobacteria
Research Methods in Arbuscular Mycorrhizal Fungi
Field Trials of Bioinoculants.