Analytical Ultracentrifugation of Polymers and Nanoparticles
E-Book
Analytical Ultracentrifugation of Polymers and Nanoparticles
Analytical ultracentifugation (AUC) is a powerful method for the characterization of polymers, biopolymers, polyelectrolytes, nanoparticles, dispersions, and other colloidal systems. The method is able to determine the molar mass, the particle size, the particle density and interaction parameters like virial coefficients and association constants. Because AUC is also a fractionation method, the determination of the molar mass distribution, the particle size distribution, and the particle density distribution is possible. A special technique, the density gradient method, allows fractionating heterogeneous samples according to their chemical nature that means being able to detect chemical heterogeneity. The book is divided into chapters concerning instrumentation, sedimentation velocity runs, density gradient runs, application examples and future developments. In particular, the detailed application chapter demonstrates the versatility and power of AUC by means of many interesting and important industrial examples. Thus the book concentrates on practical aspects rather than details of centrifugation theory.
1;Preface;7 2;List of Symbols and Abbreviations;9 3;Table of Contents;11 4;1 Introduction;14 4.1;1.1 Historic Examples of Ultracentrifugation;15 4.2;1.2 Basic Theory of Ultracentrifugation;20 4.3;1.3 Basic Experiment Types of Ultracentrifugation;25 4.4;1.4 Closing Remarks;28 4.5;References;29 5;2 Analytical Ultracentrifugation, Instrumentation;30 5.1;2.1 Ultracentrifuges;31 5.2;2.2 Rotors;38 5.3;2.3 Measuring Cells;39 5.4;2.4 Detectors;42 5.5;2.5 Multiplexer;56 5.6;2.6 Auxiliary Measurements;56 5.7;References;59 6;3 Sedimentation Velocity;60 6.1;3.1 Introduction;60 6.2;3.2 Basic Example of Sedimentation Velocity;60 6.3;3.3 Advanced Theory of Sedimentation Velocity Runs;67 6.4;3.4 Sedimentation Velocity Runs of Macromolecules to Measure AverageMand;75 6.5;3.5 Sedimentation Velocity Runs on Particles to Measure Average dp and PSD;82 6.6;3.6 Synthetic Boundary Experiments;99 6.7;References;108 7;4 Density Gradients;110 7.1;4.1 Introduction;111 7.2;4.2 Static Density Gradients;114 7.3;4.3 Dynamic Density Gradients;133 7.4;4.4 Other Types of Density Gradients;138 7.5;References;142 8;5 Sedimentation Equilibrium;144 8.1;5.1 Introduction;144 8.2;5.2 Experimental Procedure;146 8.3;5.3 Data Analysis;151 8.4;5.4 Examples;164 8.5;5.5 Further AUCMethods to Measure Molar Masses;172 8.6;References;175 9;6 Practical Examples of Combination of Methods;176 9.1;6.1 Combination of Different AUC Methods;176 9.2;6.2 Combination of AUC with Other Techniques;202 9.3;6.3 Literature Examples: AUC and Nanoparticles;225 9.4;References;225 10;7 Recent Developments and Future Outlook;227 10.1;7.1 New AUC Instrumentation and Detectors;228 10.2;7.2 New AUC Methods;234 10.3;7.3 New AUC Data Analysis;240 10.4;References;245 11;8 Subject Index;247
| ISBN | 9783540262183 |
|---|---|
| Artikelnummer | 9783540262183 |
| Medientyp | E-Book - PDF |
| Copyrightjahr | 2006 |
| Verlag | Springer-Verlag |
| Umfang | 237 Seiten |
| Sprache | Englisch |
| Kopierschutz | Digitales Wasserzeichen |
