Antibodies are indispensable tools for research, diagnosis, and therapy. Recombinant approaches allow the modification and improvement of nearly all antibody properties, such as affinity, valency, specificity, stability, serum half-life, effector functions, and immunogenicity. "Antibody Engineering" provides a comprehensive toolbox covering the well-established basics but also many exciting new techniques. The protocols reflect the latest "hands on" knowledge of key laboratories in this still fast-moving field. Newcomers will benefit from the proven step-by-step protocols, which include helpful practical advice; experienced antibody engineers will appreciate the new ideas and approaches. The book is an invaluable resource for all those engaged in antibody research and development.

Bioinformatics of Antigen-binding Sites

Analysis of Single Chain Antibody Sequences Using the VBASE2 Fab Analysis Tool
Standardized Sequence and Structure Analysis of Antibody Using IMGT®
Protein Sequence and Structure Analysis of Antibody Variable Domains
Generation of Antibody Fragments and Their Derivatives
scFv by Two-Step Cloning
Bivalent Diabodies
Generation of Single-Chain Fv Fragments and Multivalent Derivatives scFv-Fc and scFv-CH3 (Minibodies)
Miniantibodies
Generation of Stably Transfected Eukaryotic Cell Lines Producing ImmunoRNAse Fusion Proteins
Antibody-Cytokine Fusion Proteins with Members of the TNF-Family
Recombinant Immunotoxins for Treating Cancer
T Bodies
Expressing Intrabodies in Mammalian Cells
Phenotypic Knockdown with Intrabodies
Disulfide-Stabilized Fv Fragments
PEGylation of Antibody Fragments to Improve Pharmacodynamics and Pharmacokinetics
Fusion Proteins with Improved PK
In Vivo Biotinylated scFv Fragments
Bispecific Diabodies and Single-Chain Diabodies
Generation and Characterization of a Dual Variable Domain Immunoglobulin (DVD-Ig(TM)) Molecule
Isolation of Antigen-Specific Nanobodies
CDR-FR Peptides
Production of Antibody Fragments
Purification and Characterization of His-Tagged Antibody Fragments
Production of Antibody Fragments in the Gram-Positive Bacterium Bacillus megaterium
Analysis and Purification of Antibody Fragments Using Protein A, Protein G, and Protein L
Purification and Analysis of Strep-tagged Antibody-Fragments
Production of Antibodies and Antibody Fragments in Escherichia coli
Improving Expression of scFv Fragments by Co-expression of Periplasmic Chaperones
Bioreactor Production of scFv Fragments in Pichia pastoris
Expression of Antibody Fragments in Transgenic Plants
Transient Production of scFv-Fc Fusion Proteins in Mammalian Cells
Recombinant Antibody Molecules in Nanobiotechnology and Proteomics
Immunoliposomes
Targeted Polymeric Nanoparticles.-Antibody Microarrays for Expression Analysis
Evaluation of Recombinant Antibodies on Protein Microarrays Applying the Multiple Spotting Technique
Preclinical and Clinical Development
Xenograft Mouse Models for Tumour Targeting
Xenograft Mouse Models for Tumour Targeting
Imaging Tumor Xenografts Using Radiolabeled Antibodies
Human Anti-antibody Response
IP Issues in the Therapeutic Antibody Industry.