This book highlights the latest advances in the use of graphene and bio-compatible-material-decorated graphene to detect various targets (e.g. DNA, RNA, amino acids, peptides, proteins, enzymes, antigens, glucose, DA, AA, UA, ATP, NADH, gas, ions, etc.). It focuses on the specific interaction of these substances with graphene (or modified graphene) and the efficient transduction of the target recognition event into detectable signals via various techniques. Particular emphasis is given to well-designed strategies for constructing graphene-based platforms and target determination. It also covers other bio-analytical applications including cellular imaging, drug delivery and bacteria inhibition, before turning to a discussion of future challenges and prospects of graphene in bio-analytical applications. This book is intended for researchers working in the fields of analytical chemistry, nanomaterials and biomedical engineering.

Li Niu is a Professor at the State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences.

Prof. Li Niu received his BSc in 1992, MSc in 1995 from Jilin University, and his Ph.D in 1998 from Changchun Institute of Applied Chemistry, Chinese Academy of Sciences. Then he worked as a research associate at Åbo Akademi University, Finland. In 2003, he joined Changchun Institute of Applied Chemistry as a full professor of chemistry. Now he is the Director of Engineering Laboratory for Modern Analytical Techniques. He was awarded the National Science Fund for Distinguished Young Scholars in 2012. Prof. Niu has co-authored over 160 articles in a number of prestigious international journals, i.e. Angew. Chem. Int. Ed., Adv. Mater., with an h-index of 30 and more than 3500 citations. His research interests include nanomaterials electrochemistry, conducting polymers and chemosensors, and spectro-electrochemistry.

1;Preface;6 2;Contents;8 3;1 Introduction;10 3.1;Abstract;10 3.2;References;16 4;2 Graphene for DNA Biosensing;20 4.1;Abstract;20 4.2;2.1 Investigation of DNA and Graphene Binding Interaction;20 4.3;2.2 DNA Biosensing Employing Graphene Platform;23 4.3.1;2.2.1 Fluorescent Assay;23 4.3.2;2.2.2 Electrochemical Assay;26 4.3.3;2.2.3 Electrical Assay;31 4.3.4;2.2.4 Other Detection Manners;33 4.3.5;2.2.5 DNA Sequencing Through Graphene Nanopore, Nanogap, and Nanoribbon;34 4.3.6;2.2.6 DNA Cleavage;36 4.4;2.3 RNA Detection;36 4.5;References;37 5;3 Graphene for Amino Acid, Peptide, Protein, and Enzyme Detection;43 5.1;Abstract;43 5.2;3.1 Amino Acid;43 5.3;3.2 Peptide;44 5.4;3.3 Protein;45 5.5;3.4 Enzyme;48 5.6;3.5 Immunosensors;50 5.7;References;57 6;4 Graphene for Glucose, Dopamine, Ascorbic Acid, and Uric Acid Detection;64 6.1;Abstract;64 6.2;4.1 Glucose Detection;64 6.2.1;4.1.1 Catalysis by Enzymes;64 6.2.2;4.1.2 Non-enzymatic Catalysis;70 6.3;4.2 DA, AA, and UA Detection;73 6.3.1;4.2.1 DA;74 6.3.2;4.2.2 AA;77 6.3.3;4.2.3 UA;78 6.3.4;4.2.4 Simultaneous Determination of DA, AA, and UA;78 6.4;References;80 7;5 Graphene for Detection of Adenosine Triphosphate, Nicotinamide Adenine Dinucleotide, Other Molecules, Gas, and Ions;87 7.1;Abstract;87 7.2;5.1 ATP;87 7.3;5.2 NADH;90 7.4;5.3 Other Molecules, Gas, and Ions Sensing;93 7.4.1;5.3.1 Other Molecules;93 7.4.2;5.3.2 Gas;97 7.4.3;5.3.3 Ions;98 7.5;References;102 8;6 Graphene in Drug Delivery, Cellular Imaging, Bacteria Inhibition, Versatile Targets Bioassays;109 8.1;Abstract;109 8.2;6.1 Graphene Cytotoxicity, Cellular Imaging, and Drug Delivery;109 8.3;6.2 Pathogen and Bacteria Inhibition;114 8.4;6.3 Versatile Targets Bioassays;116 8.5;References;118 9;7 Conclusions and Perspectives;121 9.1;Abstract;121