The Japanese biotechnology community has developed a unique system of scientific bilateral communications at the international level. It consists of closed seminars where a restricted number of speakers from Japan and a selected country are invited. Thereby the actual state of biotechnology is visualized very effectively and valuable information exchanged during the discussions and personal contacts. As a result strong relationships between several European and American countries have been built up during recent years and numerous colleagues from abroad have improved their knowledge of Japanese science, economy, and culture. The present volume reports on an American-Japanese meeting held in Moriyama, which is situated on the lovely Lake Biwa in Japan. The well prepared and efficiently organized event covered the major aspects of bioprocess engineering including animal and plant cell culture, biocatalysts, downstream processing, sensor and bioprocess control, as well as genetic engineering. Altogether a broad spectrum, indicating the strengths and weaknesses of the current efforts being made for the improvement of process technology and the search for new products.

1. Plant and Animal Cell Culture

1. 1 Cultured Plant Cells as the Catalysts for Bioreactor
1. 2 Characteristics and Utility of Human Hematopoietic Cell Lines
1. 3 Scale-up of Oxygen Transfer in Animal Cell Cultures
1. 4 Stoichiometric Considerations of Mammalian Cell Metabolism in Bioreactors
2. Biocatalysts
2. 1 Microbial and Enzymatic Processes for the Production of Biologically Useful Compounds
2. 2 Inducible Recombinant Cell Cultures and Bioreactors
2. 3 Intradiffusion Effect on Reactivity of Immobilized Microorganisms
2. 4 The Adsorption of Trichoderma reesei C 30 Cellulase Protein to Protein Adsorbents: Application to Cellulase Recovery and Reuse
2. 5 The Construction of a Bioreactor for the Production of an Optically Active Compound
2. 6 A New Method of Graphical Analysis of Batch Data for Continuous Operation, the "Tangential Method"
2. 7 Enzymes in Organic Synthesis: Aldol Reactions as a Route to Unusual Sugars
2. 8 Production of L-Phenylalanine Using Enzymes
3. Genetic Engineering
3. 1 Genetic Engineering in Coryneform Bacteria
3. 2 Formation of Jumbo Yeast Cells by Introduction of Methylglyoxal Resistant Genes, Development of New Vector for Wild or Industrially Used Cells
3. 3 Cloning and Over-Expression of the E. coli D-Xylose Isomerase Gene in Various Microorganisms
3. 4 Production of Useful Proteins by Bacillus brevis
3. 5 Extracellular Secretion of Cloned Serratia marcescens Protease through the Outer Membrane from Escherichia coli Cells
3. 6 Cumulative Effect of Intragenic Amino Acid Replacements on Thermostability of a Protein
4. Down Stream and Integrated Processes
4. 1 Separation and Purification in Bioprocess
4. 2 Bioseparations of Milk Proteins
4. 3 Design and Use of Enzyme Systems for SelectiveProduct Release from Microbial Cells
4. 4 Dynamic In-Situ Fractionation of Extracellular Proteins Produced in a Baker's Yeast Cultivation Process
4. 5 Supercritical Fluid Bioreactor
4. 6 Application of Affinity Adsorption in Antibiotic Production
4. 7 Applications of Cross-Flow Filtration for High Density Cultivation and Regulation of trp Promoter
4. 8 Extraction of Volatile Fatty Acids from Spent Medium with a Supported Liquid Membrane
5. Sensoring and Control of Bioprocesses
5. 1 Microbiosensors for Clinical and Process Analysis
5. 2 Use of Infrared Spectroscopy for the On-Line Multicomponent Analysis and Control of Bioprocesses
5. 3 The Use of Fluorometry in Monitoring and Control of Cell Cultures
5. 4 Mass Production of Intracellular Metabolite by Fully Automatic Fed-batch Culture of Microorganism
5. 5 A Database System and an Expert System for Realizing Factory Automation in Bioindustries
5. 6 Recombinant Bioprocess Technology: Is it Different from Traditional Microbial Culture Process Technology
5. 7 Effect of Shear Stress on Xanthan Gum Production
5. 8 The Biological Component of Expert Systems.