During their lifetime, especially when growing and dividing, cells go through various steps of the cell cycle. Knowledge of the individual steps of the cell cycle will help us understand the development of a variety of diseases better, including cancer, and also to design new drugs against it. New techniques for studying the molecular basis of these processes have recently been developed and are described in detail in this manual.
A glossary helps the reader to cope with the complex cell cycle terminology.

One Techniques for Studying the Mammalian Cell Cycle

1 Use of Immunohistochemistry in Cell Cycle Studies
2 Immunofluorescence Techniques in Cell Cycle Studies
3 Fluorescence in Situ Hybridization and Human Cell Cycle Genes
4 Techniques for Monitoring Cell Cycle Phases
5 Centrifugal Elutriation
6 Synchronization of Mammalian Cells
7 Microinjection and Electroporation of Macromolecules into Live Cells
8 Stable and Transient Transfections in Cell Cycle Studies
9 Quantification of Premature and Normal Mitosis
Two Techniques for Studying the Xenopus Cell Cycle
10 Preparation of Xenopus Egg Extracts and Their Utilization in Cell Cycle Studies
Three Techniques to Study the Yeast Cell Cycle
11 Cloning and Expression of Cell Cycle Genes in Schizosaccharomyces pombe
12 Preparation of Synchronous Cultures of the Fission yeast, Schizosaccharomyces Pombe
Four Techniques for Studying the Cell Cycle of Any Species
13 Identification of Novel Cell Cycle Targets Using Small Molecule Ligands
14 Bacterial Expression and in Vitro Assay of Cell Cycle Kinases
15 Heterologous Expression and Secretion of Cell Cycle Proteins Using the Yeast Pichia pastoris
16 SV40 Based in Vitro DNA Replication Assay
17 From Sequence to Function-Structural Motifs of Cell Cycle Proteins
18 Purification of Cell Cycle Kinases
19 Interaction-Based Cloning of Novel Proteins
20 Expression of Cell Cycle Proteins Using Recombinant Baculoviruses
21 Cell Cycle-Regulated Transcription Factors: Bandshift Assays
22 Immunoprecipitation and Immunoblotting in Cell Cycle Studies
23 Protein mapping in Cell Cycle Studies
24 From Peptide to Purified Antibody.