In this volume the impact of cell culture models ondermatological research is discussed by scientists frommedicine, physiology, biology, pharmacology,and pharmacy.The book includes the following topics: orthology andpathology of the skin, differentiation of keratinocytes andfibroblasts, problems of the cultivation of melanocytes,biochemistry of pigment metabolism, models for wound healingand tissue renewal, fibroblast function and metabolism ofcollagen, models for the investigation of ageing as well asmodels for pharmacological and toxicologial tests.Thus, a wide arch is spanned, from basic problems withcultivation and characterization of cell cultures toexamples of application. Dermatologists as well as cellbiologists will benefit from this publication.

1. Models in Orthology and Pathology

Development of a Cell Culture System to Detect and Analyze Pathogenicity of Autoantibodies Directed to Nuclear Antigens
Cell Cultures of Fetal Human Skin Are Suitable Models for the Investigation of Mitogenic Activity in Psoriasis Sera
Influence of Human Sera on the Adherence of Candida albicans to Buccal and Intestinal Cells in Culture
Modulation of 1,25-Dihydroxyvitamin D3 Receptor (VDR) Expression in HaCaT Keratinocytes
Aminopeptidases of Human Skin - a Survey of Enzyme Activities in Extracts from Skin and Cultured Fibroblasts
Determination of Cell Counts and Cell Proliferation by the Fluorochromes Hoechst 33342 or Hoechst 33258
Volume Determination of Adhering Cells in Culture by Means of Acoustic Interferometry
Effects of Uremic Compounds on Cultured Myocytes: a Model for Uremic Myocardiopathy
Cultivation of Trophoblast Tissue of Human Intrauterine and Ectopic Pregnancies and Treatment with Methotrexate
2. Models in Differentiation of Keratinocytes
Mechanical Stimulation Enhances Human Keratinocyte Differentiation in Culture: Induction of Cytokeratin 9 Synthesis
N-Alkanes in Cultured Normal Human Keratinocytes
Influence of Retinoids on the Keratin Profile in Normal and Spontaneously Transformed Human Keratinocytes
Cytological Characterization of Three-dimensional, Graftable Human Keratinocytes Cultures
Fluorometric Measurement of Suprabasal Cytokeratins and DNA of Human Keratinocytes Cultivated in Microtiter Plates
3. Models in Pigmentation
Biochemistry of Mammalian Pigmentation: Enzymatic Regulation of Melanogenesis
Cultured Epidermal Melanocytes from Patients with Neurofibromatosis 1 (Recklinghausen's Disease): a Cell Culture Model for Neurofibromatosis 1
Pigmentation in Cultures ofHuman Melanocytes
4. Models in Wound Healing
Re-epithelialization of Incision and Burn Wounds in Culture
Primary Culture of Bilayered Epidermal Cell Sheets
Plasminogen Activator Contained in Secretions of a Keratinocyte Cell Line and in the Fluid of Intraepidermal Pemphigus vulgaris Blisters Is Activated by Sodium dodecyl sulfate
Are Rat Granulation Tissue Fibroblasts Regulated by Cytokines in the Same Manner as Skin Fibroblasts?
Cultured Allografts for Wound Conditioning and Enhanced Epithelialization of Chronic Venous Ulcers
Collagen Type-I Is Chemoattractive for the Human Keratinocyte Cell Line (HaCaT)
5. Models in Fibroblast Function and Collagen Metabolism
The Molecular Biology of the Terminal Differentiation of Dermal Fibroblasts in the Fibroblast Stem Cell System In Vivo and In Vitro
Cytokine-dependent Regulation of Human Dermal Fibroblasts Cultured in a Three-dimensional Collagen-Gel
Investigation of Components of the Dermal-Epidermal Junction Zone In Vitro: Effect of Transforming Growth Factor-beta on Collagen VII Expression
The Influence of Retinoids on Chemotaxis and Connective Tissue Synthesis of Fibroblasts
6. Models in Skin Ageing
In Vitro Studies of Fibroblast Aging: Growth Factors, Collagen and Glycosaminoglycan Synthesis - a Review
Terminal Differentiation, Aging, Apoptosis, or Transformation of the WI-38 Fibroblasts in the Fibroblast Stem Cell System In Vitro
Differential Gene Expression, Protein Synthesis, and Degradation in Aging Fibroblasts
Age Differences in Human Skin Collagen
Influence of Cell Density of Energy Metabolism and the Actin Skeleton of Different Cell Types
7. Models in Skin Pharmacology and Toxicology
(+)-Aeroplysinin-l: an Inhibitor or Intrinsic Protein Tyrosine Kinase Activity ofEGF-Receptor-Kinase Complex
In Vitro Cultivation of Human Dermal Microvascular Endothelial Cells as a Model for the Dermatopharmacology
Effect of Nicotine on the Differentiation of Human Keratinocytes In Vitro
A New In Vitro Test System and Phototoxicological Studies on Cell Cultures Based on the Melamine Substrate Foil
In Vitro Assays for Evaluation of the Effects of 8-Methoxypsoralen and UV Irradiation of Human Lymphocytes and Granulocytes
Comparative Cytotoxicity Test with Human Keratinocytes, HaCaT Cells, and Skin Fibroblasts to Investigate Skin-Irritating Substances
SDS-Induced Activation of the Cutaneous Plasminogen Activator System: A Molecular Pathway Possibly Involved in SDS-Induced Skin Irritation.