This atlas provides a detailed overview of the novel technique of ex vivo confocal microscopy for rapid imaging of excised tissues in dermatological practice. It features an extensive collection of ex vivo images acquired from normal skin structures and from a variety of neoplastic lesions (benign and malignant) and inflammatory lesions. Each chapter contains several image types of a particular disorder, including gray-scale, digital purple-pink images (DHE) and hematoxylin and eosin (H&E) correlations to assist the acquisition of diagnostic skills. Guidance on how to use techniques for tissue preparation, staining, handling and image acquisition are also provided enabling the reader to develop confidence in integrating this technique into their day-to-day practices. Furthermore, this atlas also provides an update on the ongoing latest advances in the field.

 

Cutaneous Atlas of Ex Vivo Confocal Microscopy covers how to apply these techniques into dermatological practice, especially in Mohs surgery for the evaluation of keratinocytic neoplasm and in dermatopathology for rapid evaluation of varied skin lesions. It is therefore a valuable resource for trainee, residents, practicing dermatologists and dermatopathologists who are seeking a resource to assist in developing their knowledge and skills of utilizing these methodologies. 

Section I. Basics of Ex Vivo Confocal Microscopy

Roles and Applications of Ex Vivo Confocal Microscopy
Principles of Ex Vivo Confocal Microscopy
Hands-On Guide for Ex Vivo Confocal Imaging
Section II. Normal Skin
Normal Skin on Ex Vivo Confocal Microscopy: Patterns and Histopathologic Correlation
Section III. Benign Skin Tumours
Features of Benign Epidermal Nonmelanocytic Lesions on Ex Vivo Confocal Microscopy and Histopathologic Correlation
Features of Benign Nonmelanocytic Dermal and Subcutaneous Lesions on Ex Vivo Confocal Microscopy and Histopathologic Correlation
Section IV. Malignant Keratinocyte Neoplasms
Morphological Features of Basal Cell Carcinoma on Ex Vivo Confocal Imaging and Histopathologic Correlation
Squamous Cell Carcinoma Features on Ex Vivo Confocal Imaging and Histopathologic Correlation
Section V. Melanocytic Lesions
Normal Nevi: Junctional, Compound, and Dermal
Dysplastic Nevi
Melanoma
Section VI. Inflammatory Lesions
Featuresof Lichen Planus and Psoriasis on Ex Vivo Confocal Imaging and Histopathologic Correlation
Eczema: Features on FCM, Digital H&E, and Corresponding Conventional H&E
Fluorescence Confocal Microscope for Identification of Discoid Lupus Erythematosus Histologic Features
Immunofluorescence with Confocal Microscopy: Pemphigoid, Pemphigus, Cutaneous Vasculitis, Lichen Planus, and Cutaneous Lupus Erythematosus
Section VII. Future of Ex Vivo Confocal Microscopy
Advances in Technology, Staining Protocol, and Flattening Devices in Ex Vivo Confocal Microscopy
Epidermal Reconstruction During Ex Vivo Confocal Microscopy for Detection of Superficial Basal Cell Carcinoma with 3D-mosaicking and Intensity Projection.