The standard protocols for the purification of all known cytoskeleton proteins are presented in this manual. Proteins are listed alphabetically and each protocol follows a common format. Thus, the manual provides a quick and easy reference to all relevant procedures for cytoskeleton protein purification.
The isolation procedure for each protein is shown in a clear flowchart, while the source of the protein, equipment and material needed, a list of suppliers, standard references, accession No. of sequences as well as further relevant facts and practical tips are given on a separate page.

A General

I User's Instructions
II General Lab Equipment
III Preparation of Buffers
IV Ammonium Sulfate Precipitation
V Chromatographic Fractionation
VI Centrifugation
VII Abbreviations
B Purification Protocols
I Actin and Actin-Associated Proteins
II Tubulin and Microtubule-Associated Proteins
III Motor Proteins
IV Intermediate Filament Proteins
V Plasmamembrane and Organelle-Associated Proteins
C Suppliers List.