DNA replication is a key event in the cell cycle. Although our knowledge is far from complete and many elusive regulatory mechanisms still remain beyondour grasp, many enzymes and a multiplicity of biochemical mechanisms involved have been discovered. Recent findings in E. coli have confirmed and yet surpassed the original hypothesis of F. Jacob. In yeast and higher eucaryotes, the apparent redundancy in putative origins and initiators has made an estimation of the importance of each identified element difficult to access. In spite of well established methodologies - which are also described in the book - the origin identification in mammalian chromosomes is still a controversial subject. On the other hand, considerable advances have been made in our understanding of virus DNA replication and this continues to deepen and broaden our understanding of the controls of cellular DNA replication.

Regulation of Initiation of Chromosome Replication

1: Cellular DNA Replication
Cell cycle control of DNA replication
1. Escherichia coli strains in which the chromosome is replicated from origins other than oriC
2. Dam methyltransferase in Escherichia coli: Effects of different enzymatic levels on DNA replication and cell growth
3. Further characterization of a DNA replication inhibitor from the cell membrane of Escherichia coli
4. The involvement of cdc2 in cell cycle control of DNA replication in Xenopus egg extracts
5. A gene of Saccharomyces cerevisiae probably involved in the coordination of chromatin replication and cell division
Origin identification in mammalian chromosomes
6. Origins of DNA replication and nucleosome segregation in mammalian chromosomes
7. Directions of DNA replication in yeast and mammalian cells
8. The curious case of the novel 2-D gel artifact
Origin structure, function and modification
9. Structural and functional characteristics of autonomously replicating mammalian origin-enriched sequences (ORS)
10. Mutations affecting replication origin function in yeast
11. Role of transcriptional elements in activating origins of replication
12. Telomere replication in Saccharomyces cerevisiae
Initiator - replication origin recognition
13. The role of DnaA-DnaA box interaction in initiation of chromosomal replication in Bacillus subtilis
14. DnaA protein mediated transcription termination in the GUA operon of Escherichia coli
15. Screens for proteins binding to the ARS consensus sequence
16. Protein-DNA interaction at yeast replication origins: an ARS consensus binding protein
17. Genetic Analysis of the roles of yeast ARS binding factor I
18. Proteins involved in ARS function in yeast
19. ARS bindingfactors from Saccharomyces cerevisiae
Initiator interactions with DNA polymerases and accessory proteins
20. Mutations within the minimal replicon of plasmid pPS10 increase its host range
21. Human type I DNA topoisomerase: Regulation of expression and function
22. Holoenzyme forms of yeast DNA polymerases ? and ?
23. The pol1 and pol3 DNA synthesis genes in fission yeast Schizosaccharomyces pombe
24. DNA Polymerase ? (POL3) of Saccharomyces cerevisiae
25. Genetic control of the DNA polymerase ?-primase complex in the yeast Saccharomyces Cerevisiae
2: Viral and Plasmid DNA Replication
Molecular interactions during protein primed initiation
26. Protein-primed replication of bacteriophage Ø29 DNA
27. Molecular interactions at the adenovirus origin of DNA replication
28. Analysis in vitro of mutations in the cloned precursor to the terminal protein (pTP) and the adenovirus DNA polymerase (ad Pol) genes
29. The role of transcription factors in adenovirus DNA replication
Molecular interactions at origins on circular viral and phage DNA
30. The replication initiator protein of P1 is activated by two E. coli heat shock proteins, DnaJ and DnaK
31. Bacteriophage Lambda DNA replication and the role of the universally conserved dnaK, dnaJ and grpE heat shock proteins
32. SV40 DNA replication with purified proteins : functional interactions among the initiation proteins
33. Bovine RP-A functions in SV40 DNA replication in vitro, but bovine polymerase ?-primase inhibits replication competitively
34. Bovine Papillomavirus (BPV1) replication protein E1: associated ATPase and binding to the viral E2 transactivator.