DNA and RNA Isolation Techniques for Non-Experts

DNA and RNA Isolation Techniques for Non-Experts

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This thorough introductory volume presents the background, applications, and stepwise directions for standard DNA and RNA isolation techniques. Unlike a kit chemistry approach, this book provides a breadth of information necessary for junior or non-expert researchers to learn and apply these techniques in their work. An accessible, indispensable how-to guide for researchers in immunology, molecular biology, zoology, forensic science, genetics, botany, neuroscience, physiology, and others.

1-Basic Idea About Nucleic Acids For Non-Experts
2-General Steps During Isolation Of DNA And RNA
3-Phenol-Chloroform DNA Isolation Method
4-RNA Isolation By The Guanidinium-Acid-Phenol Method
5-Spin Column-Based Isolation Of Nucleic Acid
6-Isolation Of Plasmid DNA By Alkaline Lysis
7-DNA Isolation By Hydrophilic Ionic Liquid Treatment
8-Lithium Chloride Based Isolation Of DNA And RNA
9-Isolation Of Bacteriophage DNA By Peg Method
10-DNA Isolation By Chelex Method
11-DNA Isolation By Lysozyme And Proteinase K
12-Salting-Out Method For Blood Samples
13-CTAB Or SDA Based Isolation Of Plant's DNA
14-DNA Extraction By Spooling Method
15-Magnetic Bead-Based Nucleic Acid Isolation
16-Density Gradient-Based Nucleic Acid Isolation
17-DNA Extraction From Agarose Gel Though Paper-Strips
18-Transformation Or Genetic Modification Of Cells/Organisms
19-Gene Cloning And Vectors
20-Polymerase Chain Reaction (PCR)
21-Southern And Northern Blotting
22-Genome Mapping
23-Applications Of DNA Sequencing Technologies For Current Research.


ISBN 978-3-030-94229-8
Artikelnummer 9783030942298
Medientyp Buch
Auflage 1st ed. 2022
Copyrightjahr 2022
Verlag Springer, Berlin
Umfang XVI, 203 Seiten
Abbildungen XVI, 203 p. 58 illus., 48 illus. in color.
Sprache Englisch