Recent progress on enzyme immobilisation, enzyme production, coenzyme regeneration and enzyme engineering has opened up fascinating new fields for the potential application of enzymes in a large range of different areas. As more progress in research and application of enzymes has been made the lack of an up-to-date overview of enzyme molecular properties has become more apparent. Therefore, we started the development of an enzyme data in formation system as part of protein-design activities at GBF. The present book" Enzyme Handbook" represents the printed version of this data bank. In future a computer searchable version will be also available. The enzymes in this Handbook are arranged according to the Enzyme Commission list of enzymes. Some 3000 "different" enzymes will be covered. Frequently enzymes with very different properties are included under the same EC number. Although we intend to give a representative overview on the characteristics and variability of each enzyme the Handbook is not a com pendium. The reader will have to go to the primary literature for more detailed information. Naturally it is not possible to cover all the numerous literature references for each enzyme (for special enzymes up to 40000) if the data re presentation is to be concise as is intended.

Pyrroline-2-carboxylate reductase

Pyrroline-5-carboxylate reductase
Dihydrofolate reductase
Methylenetetrahydrofolate dehydrogenase (NADP+)
Formyltetrahydrofolate dehydrogenase
Saccharopine dehydrogenase (NAD+, L-lysine forming)
Saccharopine dehydrogenase (NAD+, L-glutamate-forming)
D-Octopine dehydrogenase
1-Pyrroline-5-carboxylate dehydrogenase
Nicotinate dehydrogenase
Methylenetetrahydrofolate dehydrogenase (NAD+)
D-Lysopine dehydrogenase
Alanopine dehydrogenase
Ephedrine dehydrogenase
D-Nopaline dehydrogenase
Methylenetetrahydrofolate reductase (NADPH)
DELTA1-Piperideine-2-carboxylate reductase
Strombine dehydrogenase
Tauropine dehydrogenase
N5-(Carboxyethyl)ornithine synthase
Thiomorpholine-carboxylate dehydrogenase
beta-Alanopine dehydrogenase
Sarcosine oxidase
N-Methyl-L-amino-acid oxidase
N6-Methyl-lysine oxidase
(S)-6-Hydroxynicotine oxidase
(R)-6-Hydroxynicotine oxidase
L-Pipecolate oxidase
(S)-Tetrahydroprotoberberine oxidase
Reticuline oxidase
Dimethylglycine oxidase
Polyamine oxidase
Pyrimidodiazepine synthase
Electron-transferring-flavoprotein dehydrogenase
Sarcosine dehydrogenase
Dimethylglycine dehydrogenase
L-Pipecolate dehydrogenase
Nicotine dehydrogenase
Methylglutamate dehydrogenase
Spermidine dehydrogenase
Trimethylamine dehydrogenase
Proline dehydrogenase
Methylenetetrahydromethanopterin dehydrogenase
NAD(P)+ transhydrogenase (B-specific)
NAD(P)+ transhydrogenase (AB-specific)
Cytochrome-b5 reductase
NADPH-ferrihemoprotein reductase
NADPH-cytochrome c2 reductase
Leghemoglobin reductase
Cystine reductase (NADH)
Glutathione reductase (NADPH)
Protein-disulfide reductase (NAD(P)H)
Thioredoxin reductase (NADPH)
CoA-glutathione reductase (NADPH)
Asparagusate reductase (NADH)
Trypanothione reductase
Bis-gamma-glutamylcystine reductase (NADPH)
CoA-disulfide reductase (NADH)
NADH dehydrogenase (ubiquinone)
Monodehydroascorbate reductase (NADH)
Nitrate reductase (NADH)
Nitrate reductase (NAD(P)H)
Nitrate reductase (NADPH)
Nitrite reductase (NAD(P)H)
Hyponitrite reductase
Azobenzene reductase
GMP reductase
Trimethylamine-N-oxide reductase
Nitroquinoline-N-oxide reductase
Hydroxylamine reductase (NADH)
4-(Dimethylamino)phenylazobenzene reductase
N-Hydroxy-2-acetamidofluorene reductase
NAD(P)H dehydrogenase (FMN)
NADPH dehydrogenase (flavin)
NADPH dehydrogenase
NAD(P)H dehydrogenase (quinone)
NADH dehydrogenase
NADH dehydrogenase (quinone)
NADPH dehydrogenase (quinone)
Dihydropteridine reductase
Aquacobalamin reductase
Cob(II)alamin reductase
Aquacobalamin reductase (NADPH)
Cyanocobalamin reductase (NADPH, cyanide-eliminating)
Ferric-chelate reductase
Nitrite reductase (cytochrome)
Nitroethane oxidase
Acetylindoxyl oxidase
Urate oxidase
Hydroxylamine oxidase
3-aci-Nitropropanoate oxidase
Ferredoxin-nitrite reductase
Ferredoxin-nitrate reductase
Hydroxylamine reductase
Nitrite reductase
Nitrate reductase
5,10-Methylenetetrahydrofolate reductase (FADH2)
Nitrous-oxide reductase
Nitric-oxide reductase
Sulfite reductase (NADPH)
Hypotaurine dehydrogenase
Dihydrolipoamide dehydrogenase
Sulfite dehydrogenase
Thiosulfate dehydrogenase
Sulfite oxidase
Thiol oxidase
Glutathione oxidase
Methanethiol oxidase
Glutathione-homocystine transhydrogenase
Protein-disulfide reductase (glutathione)
Glutathione-CoA-glutathione transhydrogenase
Glutathione-cystine transhydrogenase
Methionine-S-oxide reductase
Protein-methionine-S-oxide reductase
Enzyme-thiol transhydrogenase (oxidized-glutathione)
Glutathione dehydrogenase (ascorbate)
Sulfite reductase (ferredoxin)
Sulfite reductase
Adenylylsulfate reductase
Hydrogensulfite reductase
Cytochrome-c oxidase
Pseudomonas cytochrome oxidase
Nitrate reductase (cytochrome)
Iron-cytochrome-c reductase
trans-Acenaphthene-1,2-diol dehydrogenase
...