Enzyme Histochemistry

A Laboratory Manual

Enzyme Histochemistry

A Laboratory Manual

106,99 €*

in Vorbereitung

Falls Sie eine Lieferung außerhalb DE, AT oder CH wünschen, nutzen Sie bitte unser Kontaktformular für eine Anfrage.

During recent years enzyme histochemical reactions have increasingly been considered as important, the reason being that enzyme histo chemistry is now a well-established link between morphology and bio chemistry. The development of numerous new methods and in particular the improvement of existing techniques contributed to the expansion of enzyme histochemical reactions. Today, the use of these methods allows detailed insight into molecular processes of single cells and their constituents. The selection of a suitable method for enzyme histochemical investigations needs thorough knowledge and critical evaluation of the reactions de scribed for the histochemical demonstration of enzymes and introduced in laboratory practice. Often, it is difficult for scientists primarily concerned with the application of methods and for laboratory assistants to comment on the value of an enzyme histochemical reaction. Our book will serve as a guide in this respect. It contains the most important histochemical methods for the localization of enzymes, all of which were checked by the authors themselves. These methods were often modified and frequently used for numerous different investigations of healthy and diseased organs in basic research and in routine practice.

A. Introduction
B. General Considerations
I. Conditions for the Histochemical Demonstration of Enzymes
II. Terminology and Classification of Enzymes
III. Principles of Reactions in Histochemical Methods for the Detection of Enzymes
IV. Artifacts and Control Reactions
V. Correlation of Histochemical and Biochemical Findings
C. Preparation of Tissues
I. Tissue Sampling
II. Further Treatment
III. Incubation
D. Detection Methods
I. Instructions
II. Hydrolases
III. Transferases
IV. Lyases
V. Oxidoreductases
E. Solutions and Buffers
F. List of Firms
G. References
H. Subject Index and Source List.
ISBN 9783540092698
Artikelnummer 9783540092698
Medientyp Buch
Copyrightjahr 1979
Verlag Springer, Berlin
Umfang 344 Seiten
Abbildungen X, 344 p. 116 illus.
Sprache Englisch