This new edition captures the advances made in the field of evolutionary systems biology since the publication of the first edition. The first edition focused on laying the foundations of evolutionary systems biology as an interdisciplinary field, where a way of thinking and asking questions is combined with a wide variety of tools, both experimental and theoretical/computational. Since publication of the first edition, evolutionary systems biology is now a well-known term describing this growing field.

The new edition provides an overview of the current status and future developments of this interdisciplinary field. Chapters highlight several key achievements from the last decade and outline exciting new developments, including an understanding of the interplay between complexity and predictability in evolutionary systems, new viewpoints and methods to study organisms in evolving populations at the level of the genome, gene regulatory network, and metabolic network, and betteranalysis and modeling techniques that will open new avenues of scientific inquiry.

Part 1. Theory and philosophy

Biology as a process and the consequences for studying it
Capturing the genotype-phenotype map with the adaptive multiscape
Evolution plays a high-dimensional game
What is next for evolutionary systems biology?
Part 2. Single celled organisms
Experimental evolution of microbial host-parasite relations
Engineering microbial communities that evolve in a spatial setting
Sharing is caring: differential gene flow in microbial communities.-Synthetic biology to study the evolution of gene regulatory networks
Evolution of stem cell differentiation
The evolution of epigenetic switches and cellular clocks
Part 3. Multicellular organisms
Measuring the genotype-phenotype map in eukaryotes
Pigment evolution in Drosophila fly species: time for theory?
Birds of a feather: using experiment and theory to understand the evolution of feather patterning
A regulatory network that explains both fish fins and mouse limbs
Quantitative system driftof the fly body plan
Part 4. Immunity and disease
Of mice, men, and immunity
Immunity as an evolving adaptive system
Cancer is a microevolutionary systemic disease
A systems approach to cancer.