List of Contents

The Standard FISH Procedure - an Introduction
1 FISH Techniques, FISH Probes and Their Applications in Medicine and Biology - An Overview
2 Microscopy and Imaging Systems
FISH on Native, Human Tissues
3 FISH on Chromosome Preparations of Peripheral Blood
4 FISH on Uncultured Blood or Bone Marrow
5 FISH on Blood or Bone Marrow Smears
6 Epithelial Cells from Buccal Smears and Urine
7 FISH on Hair Root Cells
8 Amniotic Fluid Cells - Uncultured
9 Human Sperm Cells
FISH on Archival, Human Tissues
10 Archival Cytogenetic Slides
11 Formalin-Fixed and Paraffin-Embedded Tissue Sections
12 Nucleus Extraction from Formalin Fixed/paraffin Embedded Tissue
13 Combined in Situ Nick Translation and mRNA FISH for Simultaneous Detection of Apoptosis and Expression of Apoptosis-Related Genes in Sections from Paraffin-Embedded Tissue
14 Nucleus Extraction from Cryofixed Tissue
15 Preparation of Samples for CGH by DOP-PCR from Cryofixed or Paraffin-Embedded, Microdissected Cells
16 Comparative Genomic Hybridization
Special FISH Techniques (Fiber-FISH, PNA-FISH, Co-FISH, NU-FISH)
17 High Resolution FISH of Stretched Chromosome Fibers
18 Quantitative DNA Fiber Mapping
19 PNA-FISH
20 Strand-Specific Fluorescence in Situ Hybridization: CO-FISH and COD-FISH
21 The Combined Use of Nuclease in Situ Digestion and FISH (NU-FISH)
22 DNA Breakage Detection-FISH (DBD-FISH)
23 Detection of Radiosensitivity by Means of Three-Color FISH
24 Fluorescence in Situ Hybridisation of a Complete Set of 41 Telomere Specific Probes in a Single Simultaneous Hybridisation Experiment Using the Chromoprobe Multiprobe System-T
FISH in Zoology and Virology
25 Zoo-FISH
26 FISH on Insect Cells Transfected with Heterologous DNA
27 Yeast FISH: Delineation of Chromosomal Targets in Vegetative and Meiotic Yeast Cells
28 In Situ Detection of Viral Nucleic Acids by Chemiluminescence
Multicolor FISH Applications
29 Spectral Imaging Analysisof Metaphase and Interphase Cells
30 Microdissection of Chromosomes and Reverse FISH
31 Multicolor FISH Techniques for the Detection of Inter- and Intrachromosomal Rearrangements
32 Centromere-Specific Multicolor-FISH (cenM-FISH)
33 Simultaneous Multicolor-FISH and Immunocyto-chemical Analysis of Fresh Tumor Material
34 Simultaneous Fluorescence Immunophenotyping and FISH (FICTION)
Not FISH but Almost
35 PRINS: Hybridization and Primed in Situ Labeling in One Step
36 Microarrays and DNA-Chips in the Analysis of Genetic Abberrations.