DNA and RNA fingerprinting based on arbitrarily primed PCR provides the most powerful tool for the study of genes.
The basic techniques are described in detailed protocols including each step from template preparation to fingerprint visualization. Various protocols for the basic techniques allow to choose between alternative strategies. In addition to the general techniques specific research applications of particular interest are given such as gene mapping, detection of somatic mutations, gene abnormally expressed in tumors or differentially expressed genes by RNA fingerprinting.

Overview

I DNA Extraction from Mammals
II Insect DNA Extraction Protocol
III Rapid DNA Extraction from Plants
IV Preparation of Fungal Genomic DNA for PCR and RAPD Analysis
V Extraction of Histoplasma capsulatum DNA for PCR
VI DNA Extraction from Bacterial Cultures
VII Arbitrarily Primed PCR and RAPDs
VIII Random Amplified Polymorphic DNA Assay
IX DNA Amplification Fingerprinting
X Fluorescent Detection and Analysis of RAPD Amplicons Using the ABI PRISM DNA Sequencers
XI Optimization of RAPD Fingerprinting
XII Fingerprint Tailoring
XIII Resolving DNA Amplification Products Using Polyacrylamide Gel Electrophoresis and Silver Staining
XIV Denaturing Gradient Gel Electrophoresis for Enhanced Detection of DNA Polymorphisms
XV Modified Temperature Sweep Gel Electrophoresis for the Separation of Arbitrarily Amplified DNA Fragments
XVI High Throughput Scoring of RAPD Fragments Through the Use of Dot-Blot Hybridization
XVII Recovering Amplified DNA from Silver Stained Gels
XVIII Cloning of RAPD Markers
XIX Sequencing of RAPD Markers
XX Analysis of Tumor-Specific Genetic Alterations by Arbitrarily Primed PCR
XXI Construction of Linkage Maps with RAPD Markers
XXII Pseudo-Testcross Mapping Strategy Using RAPD Markers
XXIII Estimating Nucleotide Divergence with RAPD Data
XXIV RAPD and PAUP Analysis for Microbial Screening Programs
XXV Production of Specific Probes for Microorganisms
Overview
XXVI Differential Display of Expressed mRNAs
XXVII RNA Arbitrarily Primed PCR
XXVIII Nonradioactive Differential Display of Messenger RNA
XXIX Fluorescent Differential Display: A Fast and Safe Way for Reliable Differential Display Analysis
XXX Slot Blot Hybridization Screening
XXXI How To Find and Clone the Appropriate cDNA Fragments Generated in Differential mRNA Display by Using Northern Blot for cDNA Capture
XXXII Verification of Differential Display Results by RNase Protection
XXXIII Direct Automated Sequencing of DDRT-PCR Fragments
XXXIV Ligation Linked PCR and Direct Sequencing of Differential Display Products
XXXV Differential Display PCR Fragments as Probes for cDNA Cloning
XXXVI Targeted RNA Fingerprinting
XXXVII Generation of Tumor-Specific DEST Catalogue
XXXVIII Analysis of Gene Expression in the Preimplantation Mouse Embryo Using mRNA Differential Display
XXXIX Rapid Identification of Differentially Expressed Genes in Trypanosomes.