1;Series Editor;5 2;Preface;6 3;Contents;8 4;Contributors;16 5;Part 1 Fluorescence Spectroscopy: Basics and Advanced Approaches;19 5.1;1 Basics of Fluorescence Spectroscopy in Biosciences;20 5.2;2 Pulse and Phase Fluorometries: An Objective Comparison;47 5.3;3 Non- Exponential Fluorescence of Electronically Coupled Donors Contains Distance Information;66 5.4;4 Fluorescence Nanotomography: Recent Progress, Constraints and Opportunities;73 5.5;5 Solvent Relaxation as a Tool for Probing Micro- Polarity and - Fluidity;88 5.6;6 Total Internal Reflection Fluorescence Microscopy: Applications in Biophysics;96 5.7;7 Single Molecule Spectroscopy: Basics and Applications;121 6;Part 2 Application of Fluorescence Spectroscopy to Biological Membranes;148 6.1;8 Raft Microdomains in Model Membranes as Revealed by Fluorescence Quenching;149 6.2;9 The Lateral Structure of Lipid Membranes as Seen by Fluorescence Microscopy;166 7;Part 3 Application of Fluorescence Spectroscopy to Protein Studies;176 7.1;10 Protein Dynamics and Protein Folding Dynamics Revealed by Time- Resolved Fluorescence;177 7.2;11 Time- Resolved Fluorescence and Two- Photon FCS Investigation of the Interaction of HIV- 1 Nucleocapsid Protein with Hairpin Loop Oligonucleotides;194 8;Part 4 Application of Fluorescence Spectroscopy to DNA and Drug Delivery;212 8.1;12 Fluorescence Techniques in Non- Viral Gene Therapy;213 8.2;13 Fluorescence Applications in Targeted Drug Delivery;241 9;Part 5 Fluorescence Spectroscopy in cells: FCS and Quantum Dots;255 9.1;14 Fluorescence Correlation Spectroscopy in Cell Biology;256 9.2;15 Fluorescent Quantum Dots: Properties and Applications;274 9.3;16 Heat Stress of Cancer Cells: Fluorescence Imaging of Structural Changes with Quantum Dots 605 and Alexa 488;286 10;Sachverzeichnis;299