"Provides analytical chemists and biomedical scientists with an excellent summary of progress...This is a book that can be recommended to all analytical scientists interested in fluorimetry." (Analytical Chimica Acta) "This is a useful overview and gives the nonspecialist a feeling for the advantages and limitations of the methods. Overall this book is a worthwhile read and a good source of references." (TRAC)
The book is divided into chapters on new methods, new appli- cations, fluorescence immunoassays, fluorometric analysis and fluorescence spectroscopy in biomedical sciences. Specific topics are fluorescence spectroscopy using synchrotron radiation, picosecond fluorescence spectroscopy, fluorescence microscopy, fluorescence scattering by synthetic polymers, fluorescence immunoassays, fluorescence for environmental monitoring, fluorescence in flow injection analysis, hydro-geological studies, fluorescence of proteins, lipids and membranes, cell fluorescence, calcium transients.

1. New Methods in Fluorescence Spectroscopy

1. Fluorescence Spectroscopy: Where We Are and Where We're Going
2. Interactions and Kinetics of Single Molecules as Observed by Fluorescence Correlation Spectroscopy
3. Fast Optical Imaging Techniques
4. Kinetic Studies on Fluorescence Probes Using Synchrotron Radiation
5. Dynamics and Geometry in Dimeric Flavoproteins from Fluorescence Relaxation Spectroscopy
6. Fluorescence Spectroscopy of Light Scattering Materials
7. Optical Sensors Based on Fluorescence Quenching
2. New Applications of Fluorometry
8. Fluorescence in Forest Decline Studies
9. Fluorescence Microscopy Studies of Structure Formation in Surfactant Monolayers
10. Fluorescence Lifetime Imaging and Application to Ca2+ Imaging
11. Ether Phospholipids in Membranes: Applications of Phase and Steady-State Fluorometry
12. Pyrene-Labelled Lipids as Fluorescent Probes in Studies on Biomembranes and Membrane Models
13. Optical Detection of Intracellular Ion Concentrations
3. Fluorimetric Analysis
14. Analytical Applications of Very Near-IR Fluorimetry
15. Fluorescence Detection in Flow Injection Analysis
16. Fluorescence Spectroscopy in Environmental and Hydrological Sciences
4. Fluorescence Immunoassay
17. Fluorescence Polarisation Immunoassay
18. Progress in Delayed Fluorescence Immunoassay
19. Chemiluminescence Detection in Immunochemical Techniques. Applications to Environmental Monitoring
5. Fluorescence in Biomedical Sciences
20. Fluorescence Transients in Neurobiology: Applications of Voltage Sensitive and Ion Indicator Dyes
21. Optical Monitoring of Postsynaptic Potential in the Early Embryonic Avian Brain Stem Using a Voltage-Sensitive Dye.