Foreign Gene Expression in Fission Yeast: Schizosaccharomyces pombe

Foreign Gene Expression in Fission Yeast: Schizosaccharomyces pombe

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Recombinant proteins, produced in cells or microorganisms, play an important role in basic research, medical science and the biotechnology industry. Many foreign gene expression systems using mammalian cells, E. coli, insect cells, yeast and other organisms as hosts have been developed. However, only recently has the expression system of Schizosaccharomyces pombe as a host become a subject of focus, as it is expected to be profitable for expressing foreign genes. The reason for this is that S. pombe is evolutionarily more advanced than other types of yeast. This book describes the use of S. pombe in basic research and industrial applications.

Characteristics of "host" and "vector". Fission yeast S.pombe: an attractive host for heterologous protein production
Constitution and development of expression vectors
Exploiting the thiamine biosynthetic gene nmt1 for regulated expression of heterologous DNAs in fission yeast
Examples of heterologous protein production in fission yeast
Overexpression of proteins with seven transmembrane spanning segments
Targeting and isolationof tagged membrane proteins
Expression of human cytochrome P450 form in S.pombe
Human papillomavirus capsid protein-pREP in S.pombe.
ISBN 978-3-540-63270-2
Artikelnummer 9783540632702
Medientyp Buch
Copyrightjahr 2006
Verlag Springer, Berlin
Umfang XVII, 179 Seiten
Abbildungen XVII, 179 p. 46 illus., 4 illus. in color.
Sprache Englisch