This open access book provides a comprehensive overview of the application of the newest laser and microscope/ophthalmoscope technology in the field of high resolution imaging in microscopy and ophthalmology. Starting by describing High-Resolution 3D Light Microscopy with STED and RESOLFT, the book goes on to cover retinal and anterior segment imaging and image-guided treatment and also discusses the development of adaptive optics in vision science and ophthalmology. Using an interdisciplinary approach, the reader will learn about the latest developments and most up to date technology in the field and how these translate to a medical setting. 

High Resolution Imaging in Microscopy and Ophthalmology - New Frontiers in Biomedical Optics has been written by leading experts in the field and offers insights on engineering, biology, and medicine, thus being a valuable addition for scientists, engineers, and clinicians with technical and medical interest who would like to understand the equipment, the applications and the medical/biological background.

Lastly, this book is dedicated to the memory of Dr. Gerhard Zinser, co-founder of Heidelberg Engineering GmbH, a scientist, a husband, a brother, a colleague, and a friend.

PART ONE - Breaking the Diffraction Barrier in Fluorescence Microscopy.-  High-Resolution 3D Light Microscopy with STED and RESOLFT.- PART TWO - Retinal Imaging and Image Guided Retina Treatment.- Scanning Laser Ophthalmoscopy (SLO)

Optical Coherence Tomography (OCT) -Principle and Technical Realization
Ophthalmic Diagnostic Imaging - Retina
Ophthalmic Diagnostic Imaging - Glaucoma.- OCT Angiography (OCTA) in Retinal Diagnostics.- OCT-based Velocimetry for Blood Flow Quantification
In Vivo FF-SS-OCT Optical Imaging of Physiological Responses to Photostimulation of Human Photoreceptor Cells
Two-Photon Laser Scanning Ophthalmoscope
Fluorescence Lifetime Imaging Ophthalmoscopy (FLIO).- Selective Retina Therapy.- PART THREE - Anterior Segment Imaging and Image Guided Treatment.- In Vivo Confocal Scanning Laser Microscopy
Anterior Segment OCT
Femtosecond-Laser-Assisted Cataract Surgery (FLACS)
Refractive Index Shaping - In-Vivo Optimization of an Implanted Intraocular Lens (IOL).- PART FOUR- Adaptive Optics in Vision Science and Ophthalmology.- The Development of Adaptive Optics and its Application in Ophthalmology
Adaptive Optics for Photoreceptor-Targeted Psychophysics
Compact Adaptive Optics Scanning Laser Ophthalmoscope with Phase Plates.