Hydrocarbon and Lipid Microbiology Protocols
Hydrocarbon and Lipid Microbiology Protocols
This Volume addresses the pros and cons of oligonucleotide probes, primers and primer combinations, and importantly considers how to design the best tools for the microbial taxa and/or processes being investigated. Individual chapters focus on the design of primers targeting genes that code for enzymes associated with the following functions: degradation of aromatic, aliphatic and chlorinated hydrocarbons under aerobic and anaerobic conditions, methanogenesis, methane oxidation, and the nitrogen cycle.
Hydrocarbon and Lipid Microbiology Protocols
There are tens of thousands of structurally different hydrocarbons, hydrocarbon derivatives and lipids, and a wide array of these molecules are required for cells to function. The global hydrocarbon cycle, which is largely driven by microorganisms, has a major impact on our environment and climate. Microbes are responsible for cleaning up the environmental pollution caused by the exploitation of hydrocarbon reservoirs and will also be pivotal in reducing our reliance on fossil fuels by providing biofuels, plastics and industrial chemicals. Gaining an understanding of the relevant functions of the wide range of microbes that produce, consume and modify hydrocarbons and related compounds will be key to responding to these challenges. This comprehensive collection of current and emerging protocols will facilitate acquisition of this understanding and exploitation of useful activities of such microbes.
Introduction
Current landscape of biomolecular approaches for assessing biodegradation of aromatic hydrocarbonsPrimers - bacterial genes encoding enzymes for aerobic alkane degradation
Primers - functional genes for anaerobic hydrocarbon degrading microbes
Primers - functional marker genes for methylotrophs and methanotrophs
Primers - functional genes and 16S rRNA genes for methanogens
Primers - functional genes for aerobic chlorinated hydrocarbon degrading microbes
Primers that target functional genes of organohalide-respiring bacteria
Primers - functional genes for nitrogen-cycling microbes in oil reservoirs.
McGenity, Terry J.
Timmis, Kenneth N.
Nogales, Balbina
| ISBN | 978-3-662-57057-9 |
|---|---|
| Artikelnummer | 9783662570579 |
| Medientyp | Buch |
| Auflage | Softcover reprint of the original 1st ed. 2017 |
| Copyrightjahr | 2018 |
| Verlag | Springer, Berlin |
| Umfang | X, 241 Seiten |
| Abbildungen | X, 241 p. 10 illus. in color. |
| Sprache | Englisch |
