"Imaging Cellular and Molecular Biological Function" provides a unique selection of essays by leading experts, aiming at scientist and student alike who are interested in all aspects of modern imaging, from its application and up-scaling to its development. The chapter content ranges from the basic through to complex overview of method and protocols, and there is also practical and detailed "how-to" content on important, but rarely addressed topics. This first edition features all-colour-plate chapters.

The philosophy of this volume is to provide student, researcher, PI, professional or provost the means to enter this applications field with confidence, and to construct the means to answer their own specific questions.

Considerations for Routine Imaging

Entering the Portal: Understanding the Digital Image Recorded Through a Microscope
Quantitative Biological Image Analysis
The Open Microscopy Environment: A Collaborative Data Modeling and Software Development Project for Biological Image Informatics
Design and Function of a Light-Microscopy Facility
Advanced Methods and Concepts
Quantitative Colocalisation Imaging: Concepts, Measurements, and Pitfalls
Quantitative FRET Microscopy of Live Cells
Fluorescence Photobleaching and Fluorescence Correlation Spectroscopy: Two Complementary Technologies To Study Molecular Dynamics in Living Cells
Single Fluorescent Molecule Tracking in Live Cells
From Live-Cell Microscopy to Molecular Mechanisms: Deciphering the Functions of Kinetochore Proteins
Cutting-Edge Applications and Utilities
Towards Imaging the Dynamics of Protein Signalling
New Technologies for Imaging and Analysis of Individual Microbial Cells
Imaging Parasites in Vivo
Computer-Assisted Systems for Dynamic 3D Reconstruction and Motion Analysis of Living Cells
High-Throughput/High-Content Automated Image Acquisition and Analysis
Cognition Network Technology - A Novel Multimodal Image Analysis Technique for Automatic Identification and Quantification of Biological Image Contents
High-Content Phenotypic Cell-Based Assays.