Biotechnological processes often require the use of immobilized biocatalysts. This translation of the most successful German edition introduces the theoretical background of this rapidly growing field followed by a detailed description of the various techniques of immobilizing, characterizing, and using biocatalysts. The comprehensive discussion of possible applications in industrial processes and in basic research results in a practical guide for everyone involved in these techniques. Furthermore, some selected experiments are added in order to facilitate the understanding of the theoretical reports.

Theoretical Section

1 General Principles
2 Methods of Immobilization
3 Characteristics of Immobilized Biocatalysts
4 Reactors for Immobilized Biocatalysts
5 Industrial Applications
6 Application in Analytical Procedures
7 Uses in Medicine
8 Uses in Basic Research
9 Special Developments and Trends
Practical Section
Exercise 1 Adsorptive Coupling of Invertase to Active Charcoal
Exercise 2 Ionic Binding of Catalase to CM-Cellulose
Exercise 3 Covalent Binding of Glucoamylase to a Carrier Bearing Oxirane Groups
Exercise 4 Immobilization of ß-Galactosidase by Cross-linking
Exercise 5 Alginate Entrapment of Yeast Cells and Their Co-entrapment with Immobilized ß-Galactosidase
Exercise 6 Construction and Use of a Biochemical Electrode for Essaying Glucose
Exercise 7 Spinning of ß-Galactosidase into Cellulose Acetate Fibers
Exercise 8 Immobilization of L-Asparaginase in Nylon Microcapsules
Exercise 9 Degradation of Cellulose in a Membrane Reactor
Exercise 10 Conversion of Fumaric Acid to Malic Acid in a Liquid-membrane Emulsion
Abbreviations and Symbols
Literature.