1;Preface;7 2;Contents;10 3;Part I Inclusions in Prokaryotes;12 3.1;Prokaryote Inclusions: Descriptions and Discoveries;13 3.1.1;Introduction;13 3.1.2;Technological Milestones;14 3.1.3;Inclusions: Descriptions and Discoveries;15 3.1.4;References;24 3.2;Bacterial Sulfur Globules: Occurrence, Structure and Metabolism;31 3.2.1;Introduction;32 3.2.2;Occurrence and Localization of Sulfur Globules;33 3.2.3;Relevant Chemical Structures;41 3.2.4;Chemical Structures of Bacterial Sulfur Inclusions;44 3.2.5;A Model Organism: the Sulfur Globules of;47 3.2.6;References;53 3.3;Acidocalcisomes and Polyphosphate Granules;62 3.3.1;Introduction;62 3.3.2;Acidocalcisome Structure and Chemical Composition in Bacteria;64 3.3.3;Acidocalcisome Functions;66 3.3.4;Enzymes Involved in Poly P Synthesis and Degradation;70 3.3.5;Functions of Poly P Discovered by the Manipulation;72 3.3.6;of the Expression of Genes Involved in Their Synthesis and Degradation;72 3.3.7;Conclusions;74 3.3.8;References;74 3.4;Bacterial Glycogen Inclusions: Enzymology and Regulation of Synthesis;80 3.4.1;Introduction: Is Glycogen a Reserve Storage Compound?;81 3.4.2;Physiological Functions of Glycogen;85 3.4.3;Enzymatic Reactions Leading to Glycogen Synthesis;86 3.4.4;Properties of the Glycogen Biosynthetic Enzymes;88 3.4.5;Genetic Regulation of Glycogen Synthesis in;105 3.4.6;References;107 3.5;Biogenesis and Structure of Polyhydroxyalkanoate Granules;118 3.5.1;Introduction;119 3.5.2;Metabolism of PHA in Prokaryotes;121 3.5.3;Structure of PHA Granules;128 3.5.4;Biotechnological Production and Applications of Nanoparticles of PHAs;136 3.5.5;References;138 3.6;Wax Ester and Triacylglycerol Inclusions;146 3.6.1;Introduction;147 3.6.2;Occurrence of Storage Lipids in Prokaryotes and their Chemical Structure;148 3.6.3;Metabolism of Storage Lipids in Bacteria;151 3.6.4;Structure and Morphology of Prokaryotic Lipid Inclusions;153 3.6.5;Formation of Prokaryotic TAG and WE Inclusions and PHA Granules;158 3.6.6;Structure, Formation and Protein Equipment of Eukaryotic Lipid Inclusions;163 3.6.7;Concluding Remarks and Future Perspectives;168 3.6.8;References;169 3.7;Cyanophycin- an Ideal Bacterial Nitrogen Storage Material with Unique Chemical Properties;176 3.7.1;Cyanophycin-an Ideal Bacterial Nitrogen Storage Material with Unique Chemical Properties;177 3.7.2;Cyanophycin Inclusions in Cyanobacteria and Chemotrophic Bacteria- Early Microscopic Observations and Analyses of CGP Granules;182 3.7.3;Metabolic Functions of GGP;184 3.7.4;CGP Synthetase and CGPase-the Key Enzymes of CGP Metabolism;188 3.7.5;Cyanophycin Synthesis in Recombinant Bacteria and Transgenic Plants;190 3.7.6;Biotechnological Production of CGP and Potential Technical Applications;193 3.7.7;Outlook and Perspectives;196 3.7.8;References;196 3.8;Insecticidal Protein Crystals of;203 3.8.1;Introduction;204 3.8.2;A Brief History of;205 3.8.3;Taxonomic Affiliation of;209 3.8.4;Role of Crystal Proteins in the Biology of;211 3.8.5;Control of Crystal Protein Synthesis and Crystal Assembly;214 3.8.6;Types of Parasporal Bodies and Crystal Inclusions;219 3.8.7;Genetic Recombination of Parasporal Inclusions to Improve Toxicity;231 3.8.8;Other Types of Inclusions Produced by;238 3.8.9;Future Research;239 3.8.10;References;239 3.9;Protein Inclusion Bodies in Recombinant Bacteria;245 3.9.1;1;246 3.9.2;Introduction;246 3.9.3;2;248 3.9.4;Protein Structure Determinants for Aggregation;248 3.9.5;3;252 3.9.6;Structure and Composition of IBs;252 3.9.7;4;256 3.9.8;Dynamics of IB Formation and Disintegration;256 3.9.9;5;267 3.9.10;Impact of the Production Process,;267 3.9.11;the Host and the Target Protein on Aggregation;267 3.9.12;6;273 3.9.13;Production of IBs and Downstream Functionalization;273 3.9.14;7;282 3.9.15;Outlook;282 3.9.16;References;282 4;Part II Cameos of Selected Additional Inclusions;301 4.1;Vacuoles;302 4.1.1;Phylogenetic Relationship of Sulfur Bacteria with Vacuoles;302 4.1.2;Morphology of Sulfur Bacteria with Vacuoles;302 4.1.