Ion-selective electrodes (ISEs) have a wide range of applications in clinical, environmental, food and pharmaceutical analysis as well as further uses in chemistry and life sciences. Based on his profound experience as a researcher in ISEs and a course instructor, the author summarizes current knowledge for advanced teaching and training purposes with a particular focus on ionophore-based ISEs. Coverage includes the basics of measuring with ISEs, essential membrane potential theory and a comprehensive overview of the various classes of ion-selective electrodes. The principles of constructing ISEs are outlined, and the transfer of methods into routine analysis is considered. Advanced students, researchers, and practitioners will benefit from this expedient introduction.

Introductory issues

The basics of the ISEs
Ion-selective electrode characteristics
Ionophore-based ISEs
Glass electrodes
Ion-selective electrodes with crystalline membranes
Modern trends in the ISEs theory and applications
ISE constructions
The basics of the routine analysis with ISEs.