This is the first book about the "Kenzan" method for scaffold-free biofabrication, which  does not rely on biomaterials as scaffolds to ensure correct multicellular spheroid positioning for building three dimensional construct only made from cells. The book explains the basic principles and concepts of the microneedle-based ("Kenzan") method of building surgically-implantable tissue constructs using robotic cell spheroid-based three-dimensional bioprinting, a novel technology that opens up unique opportunities for the bioengineering of tissues and organs.

• First book on the novel Kenzan method of tissue engineering;
• Explains basic concepts and applications for organ regeneration modeling;
• Introduces a unique robotic system for scaffold-free cell construction.

Introduction: The History of the Kenzan Method

Basic Review of the Kenzan Method.- Introduction of the Bio 3d Printer: The Robotic System
Blood Vessels Project
Liver.- <br>Articular Cartilage
Trachea
Peripheral Nerve
Cardio Myocyte
Diaphragm
Bio 3d <br>Printed "Mini-Organ" for Drug Testing Tools<br>