Endothelial cell biology has developed into a vibrant discipline and has become a critical instrument to study several disease processes on the cellular and molecular level. It is now widely recognized that dysfunctions of normal endothelial cell homeostasis are involved in some of the most important human diseases, including ischemic heart diseases, hypertension, atherosclerosis, tumors, diabetes, arthritis, and inflammation. Further, the increasing importance and recognition of the field of vascular biology in general requires in vitro and in vivo techniques in order to address the complex questions.

Methods in Endothelial Cell Biology is a comprehensive practical "how-to"-guide summarizing the most relevant established techniques as well as a number of new emerging techniques. Easy-to-follow reliable protocols provide a useful lab bench resource for the experienced researcher and newcomer to the field.

I: Isolation and Culture of Endothelial Cells

1 Isolation, Purification, and Culture of Human Micro- and Macrovascular Endothelial Cells
2 Isolation of Mouse Cardiac Microvascular Endothelial Cells
3 Isolation, Culture, and Differentiation of Circulating Endothelial Precursor Cells
4 Embryonic Stem Cell-Derived Endothelial Cells
5 Detection, Isolation, and Culture Of Lymphatic Endothelial Cells
6 Three-Dimensional Spheroid Culture of Endothelial Cells
7 Immortalization of Endothelial Cells
8 Viral Gene Transfer Into Endothelial Cells
II: Functional Assays in Vitro
9 Leukocyte Adhesion to Endothelium in Vitro Under Shear Stress
10 Application of Biomechanical Forces on Endothelial Cells
11 Endothelial Cell Permeability Assays in Culture
12 Three-Dimensional in Vitro Angiogenesis Assays
13 Rat Aortic Ring Assay of Angiogenesis
14 Endothelial Cell Chemotaxis and Chemokinesis Assays
15 FACS Analysis of Endothelial Cells
16 Application of the RNA Interference (RNAi) Technology to Angiogenesis Research
III: Functional Assays in Vivo
17 Murine Bone Marrow Transplantation Models that Enable the Study of EPC Recruitment
18 Balloon Denudation of Blood Vessels
19 Peripheral Hind Limb Ischemia Models
20 In Vivo Matrigel Angiogenesis Assays
21 Analysis of Vascular Permeability in vivo
22 Validating the Manipulation of Endothelial Cell signaling in Vivo
23 Chick Chorioallantoic Membrane Assay: Growth Factor and Tumor-induced Angiogenesis and Lymphangiogenesis
24 Corneal Angiogenesis Assay
IV: Detection of Endothelial Cells
25 Basic Blood Vessel Immunohistochemistry
26 Double-Staining Immunohistochemistry of Blood Vessels
27 In Situ Hybridization Analysis of Vascular Endothelium
28 High-Resolution in Situ Confocal Analysisof Endothelial Cells
29 Whole Mount Analysis of the Embryonic Vasculature
30 Methods for Visualizing Intact Blood Vessels
31 Intravital Multi-Fluorescence Microscopy
32 Corrosion Cast Analysis of Blood Vessels
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