5 Function of Splenic Compartments . . . . . . . . . . . . . . . 45 5. 1 Splenic White Pulp Compartments during Primary T Cell-Dependent Antibody Responses against Protein Antigens . . . . . . . . . . . . . . . . . . . . . . . . . 46 5. 1. 1 Priming of CD4+ Helper T Cells by Dendritic Cells in the PALS . . . . . . . . . . . . . . . . . . . . 46 Summary . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 49 5. 1. 1. 1 5. 1. 2 Interaction of Primed CD4+ T Cells with Antigen-Specific B Cells in the PALS and Formation of Extrafollicular Foci . . . . . . . . . . . . . . . . . . . . . . . . . . . 49 5. 1. 2. 1 Summary . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50 5. 1. 3 Formation of Germinal Centres . . . . . . . . . . . . . . . . . . . 50 5. 1. 3. 1 Summary . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 55 5. 1. 4 Localisation of Memory B Cells in the Marginal Zone . . . . . . . . . . . . . . . . . . . . . . . . . . . . 55 5. 1. 4. 1 Summary . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 57 5. 2 Function of the Marginal Zone during Primary Antibody Responses against T Cell-Independent Type 2 Antigens . . . . . . . . 57 5. 2. 1 Summary . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 59 Function of the Red Pulp . . . . . . . . . . . . . . . . . . . . . . . . . 59 5. 3 5. 3. 1 Summary . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 61 5. 4 Role of the Spleen in CD8+ Cytotoxic T Cell Responses . . . . . . . . . . . . . . . 61 5. 4. 1 Summary . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62 The Spleen, Natural Killer Cells 5. 5 and Gamma/Delta T Cells . . . . . . . . . . . . . . .. . . . . . . . . 62 5. 5. 1 Summary . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 63 6 Recirculation of Lymphocytes Through the Spleen . . 65 6. 1 Summary . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 67 7 The Role of Cytokines and Chemokines in the Development of Splenic Compartments . . . . . . 69 7. 1 Summary . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 72 8 Unsolved Problems of Human Splenic Structure and Function . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 73 VI 8. 1 Arterial Blood Supply to the Splenic Follicles and to the Perifollicular Zone. . . . . . . . . . . . . . . . . 73 . . . . 8. 1. 1 Summary . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 74 8.

1 Introduction

2 Materials and Methods
2.1 Animal Spleens
2.2 Human Spleens
2.3 Antibodies
2.4 Single Staining Procedure for Immunohistology
2.5 Double Staining Procedure for Immunohistology
2.6 Demonstration of Acid Phosphatase in Cryostat Sections
2.7 Demonstration of Alkaline Phosphatase in Cryostat Sections
3 Microanatomical Compartments of the Rat Spleen
3.1 White Pulp
3.2 Red Pulp and Splenic Vessels
3.3 Summary
4 Microanatomical Compartments of the Human Spleen
4.1 White Pulp
4.2 Red Pulp and Splenic Vessels
4.3 Summary
5 Function of Splenic Compartments
5.1 Splenic White Pulp Compartments during Primary T Cell-Dependent Antibody Responses against Protein Antigens
5.2 Function of the Marginal Zone during Primary Antibody Responses against T Cell-Independent Type 2 Antigens
5.3 Function of the Red Pulp
5.4 Role of the Spleen in CD8+ Cytotoxic T Cell Responses
5.5 The Spleen, Natural Killer Cells and Gamma/Delta T Cells
6 Recirculation of Lymphocytes Through the Spleen
6.1 Summary
7 The Role of Cytokines and Chemokines in the Development of Splenic Compartments
7.1 Summary
8 Unsolved Problems of Human Splenic Structure and Function
8.1 Arterial Blood Supply to the Splenic Follicles and to the Perifollicular Zone
8.2 Blood Circulation in the Splenic Red Pulp: Subpopulations of Fibroblasts and their Role
8.3 Function of Sheathed Capillaries
8.4 Lymphocyte Migration in the Human Splenic White Pulp - A Hypothesis
9 Summary
References.