This book provides a state-of-the-art approach to the molecular basis of hematologic diseases and its translation into improved diagnostics and novel therapeutic strategies. Several representative hemato-oncologic malignancies are analyzed in detail: acute lymphoblastic leukemia, acute myeloid leukemia, B-cell Non-Hodgkin lymphomas, multiple myeloma, chronic lymphocytic leukemia, chronic myeloid leukemia, myelodysplastic syndromes, and myeloproliferative neoplasms. Experts in the field describe the molecular methods applied for modern diagnostics and therapies, such as hematopoietic stem cell transplantation, donor recipient matching, banking of biological material, analyses of post-transplant chimerism, and minimal residual disease monitoring. The volume concludes with an extensive section comprising thorough step-by-step protocols of molecular techniques in hematology, all of them validated in the authors' own laboratories.

<p>This book consists of 29 chapters and is divided into the following four Parts: Part I Molecular biology of Selected Hemato-oncologic Diseases

'Part I MOLECULAR BIOLOGY OF SELECTED HEMATO-ONCOLOGIC DISEASES<br /><br />1. Molecular biology of acute lymphoblastic leukemia<br /><br />Malgorzata Dawidowska, Monika D. Kraszewska, Katarzyna Derwich, and Tomasz Szczepanski<br /><br />2. Genetic mechanisms and molecular markers of neoplastic transformation in acute myeloid leukemia<br /><br />Agata A. Filip, Marta Libura, Sebastian Giebel, and Olga Haus<br /><br />3. Molecular pathogenesis of aggressive B-cell lymphomas<br /><br />Przemyslaw Juszczynski and Krzysztof Warzocha<br /><br />4. Molecular biology and classification of multiple myeloma<br /><br />Anna Dmoszynska and Norbert Grzasko<br /><br />5. Chronic lymphocytic leukemia<br /><br />Anton W. Langerak and Yorick Sandberg<br />6. The molecular biology of chronic myeloid leukemia<br /><br />Tomasz Sacha, Kajetana Foryciarz, and Aleksander B. Skotnicki<br /><br />7. Molecular biology of myelodysplastic syndromes<br /><br />Francesc Solé<br /><br />8. Myeloproliferative neoplasms<br /><br />Andrzej Hellmann, Maria Bieniaszewska, Witold Prejzner, and Aleksandra Leszczynska<br /><br />Part II DIAGNOSTICS AND MONITORING OF THERAPY OF HEMATO-ONCOLOGIC DISEASES<br /><br />9. Flow cytometric immunophenotyping as diagnostic tool of hematopoietic malignancies<br /><br />Lukasz S_dek, Juan Flores-Montero, Joanna Bulsa, Susana Barrena, Julia Almeida, Alberto Orfao, and Tomasz Szczepanski<br /><br />10. Cytogenetics in hematology<br /><br />Olga Haus, Anna Poluha, and Katarzyna Skonieczka<br /><br />11. Monitoring of minimal residual disease in acute lymphoblastic leukemia<br /><br />Tomasz Szczepanski, Malgorzata Dawidowska, and Katarzyna Derwich<br /><br />12. Gene expression profiling in hematologic malignancies<br /><br />Przemyslaw Juszczynski, Bjoern Chapuy, Malgorzata Szczepaniak, and Krzysztof Warzocha<br /><br />Part III MOLECULAR PROCEDURES INVOLVED IN HEMATOPOIETIC STEM CELL TRANSPLANTATION<br /><br />13. Allogeneic transplantation of hematopoietic stem cells<br /><br />Jacek Wachowiak<br /><br />14. Genetic basis of donor-recipient matching in allogeneic transplantation<br />of hematopoietic stem cells<br /><br />Jacek Nowak and Jacek Wachowiak<br /><br />15. Chimerism following allogeneic transplantation of hematopoietic stem cells<br /><br />Malgorzata Dawidowska, Katarzyna Guz, Ewa Brojer, Jacek Wachowiak, and<br /><br />Michal Witt<br /><br />16. Peritransplantation monitoring of minimal residual disease in acute lymphoblastic leukemia<br /><br />Malgorzata Dawidowska, Katarzyna Derwich, and Tomasz Szczepanski<br /><br />17. Biobanks of cellular material<br /><br />Wieslaw Wiktor-Jedrzejczak and Elzbieta Urbanowska<br /><br />Part IV MOLECULAR DIAGNOSTIC PROTOCOLS<br /><br />18. Biobanking of cellular material<br /><br />Anna Poluha and El_bieta Urbanowska<br /><br />19. Isolation of mononuclear cells from human blood and bone marrow by density gradient centrifugation<br /><br />Malgorzata Dawidowska<br /><br />20. Molecular techniques commonly used in hematooncolology<br /><br />Monika D. Kraszewska and Ewa Zi_tkiewicz<br /><br />21. Genetic methods of HLA typing<br /><br />Jacek Nowak, Renata Mika-Witkowska, and El_bieta Graczyk-Pol<br /><br />22. Post-transplant chimerism analysis through STR-PCR and RQ-PCR<br /><br />Malgorzata Dawidowska, Katarzyna Guz, Agnieszka Orzinska, Justyna Smolarczyk-Wodzynska, Monika D. Kraszewska, and Michal Witt<br /><br />23. Analysis of minimal residual disease with the use of rearrangements of Ig/TCR genes<br /><br />through RQ-PCR<br /><br />M. Dawidowska, Vincent H. J. van der Velden, Michal Witt, and Tomasz Szczepanski<br /><br />24. Molecular diagnostics of acute myeloblastic leukemia<br /><br />Marta Libura, Agata A. Filip, and Olga Haus<br /><br />25. Assessment of the presence and the level of BCR-ABL fusion gene expression and mutational status in ABL kinase domain.<br /><br />Sylwia Czekalska, Magdalena Zawada, and Izabela Florek<br />26. Studies of rearrangements and somatic hypermutation of IGHV genes in chronic lymphocytic leukemia<br /><br />Anton W. Langerak, Richard Rosenquist, Paolo Ghia, Chrysoula Belessi, Kostas Stamatopoulos, and Frederic Davi for the ERIC-IgCLL group<br /><br />27. Molecular biology methods in the diagnosis of multiple myeloma<br /><br />Anna Dmoszynska and Sylwia Chocholska<br /><br />28. Molecular methods in myeloproliferative neoplasms<br /><br />Aleksandra Leszczynska, Witold Prejzner, Maria Bieniaszewska, and