The study of membrane traffic in reconstituted cell-free systems has generated an unprecedented amount of new information on the biochemistry, molecular biology and genetics of membrane-based molecular events that underly normal and abnormal cellular function. Many of the individual steps have now been isolated and dissected in simple systems that permit detailed molecular analyses of transport mechanisms and their regulation. Reconstituted events of intercompartment transport include inter-membrane recognition, and controlled membrane fusion-fission reactions. Among the many advances is the growi ng awareness of a remarkabl e evolutionary conservation of many of the components involved in the many steps of membrane traffic, this realization has accelerated greatly the pace of progress in the field. This book provides a collection of participant contributions from the 1992 Summer Research Conference, "Mol ecul ar Mechani sms of Membrane Traffi c, " jointly sponsored with NATO by the American Society of Cell Biology. The conference was held May 9-13, at the Airlie Conference Center in the Virginia countryside, near Warrenton. The conference was attended by 158 scientists. A unique feature was the high proportion of young scientists among the participants. Approximately 65% were students, postdoctoral fe 11 ows and young investigators. Each attendee contri buted to the conference with either a pl atform or poster presentation.

Cell-Free Analysis of ER to Golgi Apparatus Vesicular Traffic

Identification of a Novel Post-ER, Pre-Golgi Compartment Where Unassembled Monomers of Oligomeric Proteins Accumulate
G Protein Regulation of Vesicular Transport Through the Exocytic Pathway
Fusion Rapidly Follows Vesicle Transport to the Target Membrane in Protein Transport Through the Golgi Apparatus In Vitro. A Reevaluation of Transport Kinetics Based on the Finding That the Glycosylation Used to Mark Transport, and Not Transport Itself is Rate Limiting in the Assay
Immunocytochemical Analysis of the Transfer of Vesicular Stomatitis Virus G Glycoprotein from the Intermediate Compartment to the Golgi Complex
Synthesis of Glycosyl-Phosphatidylinositol Anchor is Initiated in the Endoplasmic Reticulum
Protein Trafficking Along the Exocytotic Pathway
A Short N-Terminal Sequence is Responsible for the Retention of Invariant Chain (Ii) in the Endoplasmic Reticulum
Isolation and Characterization of the Principal ATPase of Transitional Elements of the Endoplasmic Reticulum of Rat Liver
A GTP Hydrolase Activity Purified from Transitional Endoplasmic Reticulum of Rat Liver Binds Retinol
A 38 kDa Protein Resident to Cis Golgi Apparatus Cisternae of Rat Liver is Recognized by an Antibody Directed Against ? Subunits of Trimeric G-Proteins
Acyl Transfer Reactions Associated with Cis Golgi Apparatus
Hepatic Bile Formation: Intracellular Trafficking of Bile Salts
Wheat Storage Proteins as a Model System to Study the Mechanism of Protein Sorting Within the Endoplasmic Reticulum
Determinants of Outer Membrane Protein Sorting and Topology in Mitochondria
Molecular Chaperones HSP70 and HSP60 in Protein Folding and Membrane Translocation
Cytosolic Reactions in Mitochondrial Protein Import.-Human Bifunctional Enzyme and Its Import Into Peroxisomes
Transport of Microinjected Proteins into the Peroxisomes of Mammalian Cells
Isolation and Characterization of a Functionally Active Protein Translocation Apparatus from Chloroplast Envelopes
Protein Insertion into the Outer Mitochondrial Membrane
The Membrane-Bound 95 kDa Subunit of the Yeast Vacuolar Proton-Pumping ATPase is Required for Enzyme Assembly and Activity
The Traffic of Molecules Across the Tonoplast of Plant Cells
Fusicoccin Binding and Internalization by Soybean Protoplasts
TGN38 and Small GTP Binding Proteins are Part of a Macromolecular Complex in the Trans-Golgi Network
?-Cop, a Coat Protein of Nonclathrin-Coated Vesicles of The Golgi Complex, is involved in Transport of Vesicular Stomatitis VirusGlycoprotein
Defining the Retention Signal in a Model Golgi Membrane Protein
Organization of the Glycoprotein and Polysaccharide Synthetic Pathways in the Plant Golgi Apparatus
CLIP-170, a Cytoplasmic Linker Protein Mediating Interaction of Endosomes with Microtubules
Maturation of Secretory Granules
Fatty Acylation in Membrane Trafficking and During Mitosis
Rates of Synthesis and Selective Loss Into the Bile of Four Rat Liver Proteins and the Polymeric IgA Receptor
Effect of Brefeldin A Treatment on the Resident Golgi Protein, MG160
Inhibition of Cholera Toxin by Brefeldin A
Selective Association with Golgi of ADP-Ribosylation Factors, 20-kDa Guanine Nucleotide-Binding Protein Activators of Cholera Toxin
Intracellular Transport and Post-Translational Modifications of a Secretory Heat Shock Protein of Saccharomyces Cerevisiae
Transport to the Cell Surface of Chlamydomonas: Mastigonemes as a Marker for the Flagellar Membrane
Lipid Transport From the Golgi to thePlasma Membrane of Epithelial Cells
Lipid Traffic to the Plasma Membrane of Leek Cells. Sorting Based on Fatty Acyl Chain Length
Hexadecylphosphocholine as a Useful Tool for Investigating Phosphatidylcholine Biosynthesis and Sorting
Biosynthesis of the Scrapie Prion Protein in Scrapie-Infected Cells
Protein Phosphorylation Regulates the Cellular Trafficking and Processing of the Alzheimer Beta/A4 Amyloid Precursor Protein
Targeting of the Polymeric Immunoglobulin Receptor in Transfected PC12Cells
Signals for Receptor-Mediated Endocytosis
Regulation of Early Endosome Fusion In Vitro
Adaptins and Their Role in Clathrin-Mediated Vesicle Sorting
Endosomes and Cell Signalling
The Tubular Early Endosome
Direct Comparision of the Endocytic Routes of Fluorescently-Labelled Lipids and Transferrin
Linkage of Plasma Membrane Proteins with the Membrane Skeleton: Insights Into Functions in Polarized Epithelial Cells
Cell Specificity and Developmental Variation in the Targeting Pathways of FRT Cells
Phosphoglucomutase is a Cytoplasmic Glycoprotein Implicated in the Regulated Secretory Pathway
Protein Targeting and the Control of Cl? Secretion in Colonic EpithelialCells
Apical Membrane Protein Sorting is Affected by Brefeldin A
Structural Analysis of the Clathrin Triskelion
Polarity in Neurons and Epithelial Cells: Distribution of Endogenous and Exogenous Ion Pumps and Transporters
Biochemical Properties and Expression of Tau Proteins in the Endocytic Compartment During Liver Regeneration
Identification of Hepatic Endocytic Proteins Potentially Involved in Membrane Traffic
Studies on Sequence Requirements for Basolateral Targeting of the Polymeric Immunoglobulin Receptor in MDCK Cells
Endocytosis by Trypanosoma Brucei: Proteases and TheirPossible Role in the Degradation of Anti-VSG Antibodies
Receptor Mediated Endocytosis of EGF in a Cell Free System
Transferrin on the Basolateral Surface Regulates Apical 59Fe Uptake and Transport Across Intestinal Epithelial Cells
Intracellular Trafficking of Salmonella typhimurium Within HeLa EpithelialCells
Functional Analysis of Dynamin, a GTPase Meditating Early Endocytosis
Identification of a Regulatory Domain in Dynamin
Potassium Depletion Stimulates Clathrin-Independent Endocytosis in Rat Foetal Fibroblasts
Clathrin Interaction with NADH Dehydrogenases of Rat Liver Plasma Membrane
Putative Adaptor Proteins of Clathrin Coated Vesicles from Developing Pea
Uncoating of Plant Clathrin Vesicles by Uncoating ATPase from Peas
Selective Degradation of Cytosolic Proteins by Lysosomes
Role of Calcium, Protein Phosphorylation and the Cytoskeleton in HepatocyticAutophagy
Signals for Transport From Endosomes to Lysosomes
A New Receptor for Lysosomal Proenzymes
A Protein Kinase/Lipid Kinase Complex Required for Yeast Vacuolar ProteinSorting
The Morphology But Not the Function of Endosomes and Lysosomes is Affected by Brefeldin A
Purification of the N-Acetylglucosamine-I-Phosphodiester ?-N-Acetylglucosamindase from Human Lymphoblasts
Altered Regulation of Protein Degradation in Tranformed Human Bronchial Epithelial Cells
An Examination of the Structure and Functions of the Bovine Vacuolar ATPase Using Antisense Oligonucleotides
Regulation of Endocytosis by the Small GTP-ase Rab5
The Nucleotide Cycle of Sec4 is Important for its Function in VesicularTransport
Activators of Trimeric G-Proteins Stimulate and Inhibit Inter-compartmental Golgi Transport In Vitro
Evidence of a Role for Heterotrimeric GTP-Binding Proteins and ARF inEndosome Fusion
The Small GTPase Rab4 Controls an Early Endocytic Sorting Event
Protein Modifications and Their Significance in rab5 Function
Low Molecular Weight GTP-Binding Proteins in Rough Endoplasmic Reticulum Membranes From Rat Liver and Rat Hepatocellular Carcinomas
Low Molecular Weight GTP-Binding Proteins Associated with the Membranes Involved in Post-Golgi Transport of Rhodopsin
Authorindex.