Molecular and Cellular Enzymology addresses not only experienced enzymologists but also students, teachers and academic and industrial researchers who are confronted with enzymological problems during their fundamental or applied research. In this field there is an urgent need for training in order to meet the requirements of both research and industrial endeavours. This book consists of several levels. Practical aspects and elementary explanations are given for the benefit of non-specialists' and students' understanding. In order to facilitate the task of students, two typographies have been adopted. The main text corresponds to basic knowledge, whereas text in a smaller font provides more specialised information. Specialists will also find topics more deeply expounded with the principal bibliographic references cited. The bibliography, however, is not exhaustive; the choice includes general books and review articles as well as some specialised articles. In this book, for the first time, the different molecular and cellular aspects of enzymology are presented together. Until now, there has been no book available in which these different aspects are treated in the same volume. In addition, besides the theoretical developments, this book provides a wealth of practical information for experimentalists.

Thermodynamic laws Notion of chemical equilibrium

Equilibrium of protein ligands association The living organisms, open systems
Chemical kinetics
Experimental methods to study the enzyme reactions Origin of enzymes and evolution
Formation of the functional structure of enzymes : co- and post-translational events
Topology of the active center of enzymes
Formation of the enzyme-substrate complexes
Catalytic mechanisms
Examples of structure-function relationships in enzyme systems
Regulation through non-covalent interactions
Covalent regulations
Multifunctional enzymes, multienzyme complexes and metabolic channeling Cellular localisation and compartmentation
Kinetics of enzyme reactions catalyzed by immobilized enzymes
Theory of the control of metabolic pathways.