The thyroid gland first appears in the phylogenic scale in the Lamprey larva, Ammocoetes, at the time of metamorphosis (see review by Constantinescu, 1972). In higher Vertebrates the adult thyroid gland consists of vesicles i. e. thyroid follicles containing colloid and lined with a cubic or prismatic epithelium. Since the end of the 19th century, many authors have studied the morphoge nesis of the follicles during the embryonic and fetal development of the gland in Man and other species, principally Chick, Rat and Rabbit. The development of techniques for culturing organs of higher animals, in particular the thyroid by Carrel and Burrows (1910) and Champy (1914, 1915), allowed the study of the survival in vivo or in vitro of grafts or explants of thyroid gland obtained from adult or fetal animals. In addition to organotypic cultures, techniques for culturing cell suspensions obtained by enzymatic dissociation have recently been refined. Moreover, histological examination of pathological human glands and adult thyroids experimentally stimulated by thyrotropin hormone (TSH) has provided additional data for the understanding of thyroid follicle morphogenesis.

Material and Methods

Cultures
Electron Microscopy
Results
I. Freshly Dissociated Cells
II. Unreassociated Cells
III. The Reassociated Cells
IV. Other Elements in the Culture
Discussion
I. Biochemical Aspects of Follicular Reassociation in Cultured Thyroid Cells
II. Ultrastructural Aspects of Follicular Reassociation
III. The Origin of the Follicular Lumen
IV. Comparative Arguments Supporting the Extracellular Origin of the Follicular Lumen
Summary
Acknowledgements
References.