A wide variety of hormones, neurotransmitters and growth factors exert their cellular effects by reacting first with membrane receptors resulting in an increase of intracellular calcium and the cellular response. The calcium signal in the cell is mediated by the high-affinity calcium binding proteins (characterized by the EF-hand structural element), and by the calcium and phospholipid dependent proteins. Many of these have been discovered most recently. Their purification, distribution, protein and gene structures as well as their physiological roles are discussed. The book is of interest to biochemists and molecular biologists as well as to clinicians and the pharmaceutical industry who can apply the results in this field.

Preface

Calcium-Binding Proteins: Past, Present, and Future
Acknowledgments
Section I Calcium Signaling by Calcium-Binding Proteins
1 The EF-Hand, Homologs and Analogs
2 Calcium-Binding Proteins of the EF-Hand-Type and of the Annexin Family: A Survey
3 Stains-All is a Dye that Probes the Conformational Features of Calcium Binding Proteins
Section II EF-Hand Calcium-Binding Proteins: The S-100 Protein Family
4 The S-100 Protein Family: A Biochemical and Functional Overview
5 p9Ka, a Calcium-Ion-Binding Protein of Cultured Myoepithelial Cells
6 S-100-Related Proteins in Nerve Growth Factor-Induced Differentiation of Pc12 Cells
7 p11, a Member of The S-100 Protein Family, Is Associated With the Tyrosine Kinase Substrate P36 (Annexin H)
8 Calcyclin - From Gene to Protein
9 Cloning and Characterization of a Cdna Encoding a Highly Conserved Ca2+-Binding Protein, Identified By An Anh-Prolactin Receptor Antibody
10 The Calgranulins, Members Of The S-100 Protein Family: Structural Features, Expression, and Possible Function
11 Mrps and Mrp 14, Two Calcium-Binding Proteins Expressed During Myelopoiesis
12 Distribution of the Calcium-Binding Proteins Mrp-8 and Mrp-14 in Normal And Pathological Conditions: Relation to the Cystic Fibrosis Antigen
Section III Novel Members of the EF-Hand Calcium-Binding Protein Family in Normal and Transformed Cells
13 Calretinin
14 Purification, Identification And Regional Localization of a Brain-Specific Calretinin-Like Calcium-Binding Protein (Protein 10)
15 Lsp1 is a New Lymphocyte-Specific Ca2+-Binding Protein Which Co-Caps With Surface Immunoglobulin
16 Nonmuscle ?-Actinin Is An EF-Hand Protein
17 EF-Hands Calcium Binding Regulates The Thioredoxin Reductase/Thioredoxin Electron Transfer In HumanKeratinocytes And Melanoma
18 Calcium-Binding Crystallins
19 Cloning and Sequencing of a New Calmodulin Related Calcium-Binding Protein Controlled by Cyclic Amp-Dependent Phosphorylation in the Thyroid: Calcyphosine
20 A 22 Kda Calcium-Binding Protein, Sorcin, is Encoded by Amplified Genes in Multidrug-Resistant Cells
Section IV Calcium-Binding Proteins in Bacteria, Fungi, and Invertebrates. Structure, Function, and Evolution
21 Coordination and Stability of Calcium-Binding Site: D-Galactose/D-Glucose-Binding Protein of Bacterial Active Transport and Chemotaxis System
22 Calcium-Binding Sites in Myeloperoxidase and Lactoperoxidase
23 A Development-Specific Ca2+-Binding Protein From Myxococcus Xanthus
24 Unique Ca2+-Binding Proteins in Metazoan Invertebrates
25 Spec Proteins: Calcium-Binding Proteins in the Embryonic Ectoderm of Sea Urchins
26 Calcium-Binding Proteins From Tetrahymena
27 Sequence And Possible Function Of A Novel Ca2+-Binding Protein Encoded In The Shaker-Locus Of Drosophila
28 A Novel EF-Hand Ca2+-Binding Protein is Expressed in Discrete Subsets of Muscle and Nerve Cells of Drosophila
Section V calcium Fluxes, Binding, and Metabolism
29 Putative Calcium-Binding Sites in Voltage-Gated Sodium and Calcium Channel ?1 Subunits
30 Alterations of Cellular Calcium Metabolism and Calcium Binding in Hypertension and Human Cardiac Hypertrophy
Section VI Calcium-Dependent- and Phospholipid-Binding Proteins in Health and Disease
31 Diversity in the Annexin Family
32 Annexin I And Its Biochemical Properties
33 The Anti-Inflammatory Activity of Human Recombinant Lipocortin 1
Author Index.