This manual provides all relevant protocols for basic and applied plant cell and molecular technologies, such as histology, electron microscopy, cytology, virus diagnosis, gene transfer and PCR. Also included are chapters on laboratory facilities, operation and management as well as a glossary and all the information needed to set up and carry out any of the procedures without having to use other resource books.
It is especially designed for professionals and advanced students who wish to acquire practical skills and first-hand experience in plant biotechnology.

Facilities and Technology Requirements for Laboratory Operations

Laboratory Facilities, Operation, and Management
Media Preparation and Handling
Sterile Techniques
Basic Plant Regeneration and Plant Propagation Procedures
Micropropagation by Proliferation of Axillary Buds
Adventitious Shoot Proliferation
Plant Regeneration by Organogenesis from Callus and Cell Suspension Cultures
Plant Regeneration from Callus and Cell Suspension Cultures by Somatic Embryogenesis
Direct Somatic Embryogenesis
Conifer Somatic Embryogenesis, Embryo Development, Maturation Drying, and Plant Formation
Applications of Organ Culture Systems
Meristem Culture for Virus Elimination
Embryo Culture and Embryo Rescue for Wide Cross Hybrids
Anther Culture for Doubled Haploid Production
Microspore Culture for Haploid Plant Production
Protoplasts and Transformation Procedures
Protoplast Isolation and Culture
Agrobacterium-Mediated Transformation
Direct DNA Transfer into Intact Plant Cells Via Microprojectile Bombardment
Microtechniques and Analytical Procedures
Botanical Microtechnique for Plant Cultures
Transmission and Scanning Electron Microscopy for Plant Protoplasts, Cultured Cells and Tissues
Indirect Immunofluorescence Techniques for Cultured Plant Cells and Protoplasts
Staining Procedures for Chromosome Analysis
Enzyme-Linked Immunosorbent Assay (ELISA) Methods to Certify Pathogen (Virus)-Free Plants
Applications of Polymerase Chain Reaction (PCR) to Plant Genome Analysis.