1;Preface;5 2;Contents;7 3;Contributors;13 4;Protein Adsorption Kinetics: Influence of Substrate Electric Potential;16 4.1;1.1 Introduction;16 4.2;1.2 Theoretical Prediction;17 4.3;1.3 Experimental Measure;21 4.4;1.4 Results;24 4.5;1.5 Discussion;32 4.6;1.6 Conclusions;36 4.7;References;36 5;From Kinetics to Structure: High Resolution Molecular Microscopy;38 5.1;2.1 Introduction;38 5.2;2.2 OpticalWaveguide Lightmode Spectroscopy;40 5.3;2.3 The Practical Determination ofWaveguide Parameters;49 5.4;2.4 Static Structure;52 5.5;2.5 Kinetic Analysis and Dynamic Structural Inference;52 5.6;2.6 Behaviour of Real Proteins;58 5.7;2.7 Conclusions;62 5.8;References;63 6;Initial Adsorption Kinetics in a Rectangular Thin Channel, and Coverage- Dependent Structural Transition Observed by Streaming Potential;66 6.1;3.1 Introduction;66 6.2;3.2 The Initial Adsorption Constant and its Limit Expressions;71 6.3;3.3 The Structural Transition with Increasing Interfacial Concentration;78 6.4;3.4 Conclusion;82 6.5;Appendix;83 6.6;References;84 7;Dual Polarisation Interferometry: An Optical Technique to Measure the Orientation and Structure of Proteins at the Solid- Liquid Interface in Real Time;90 7.1;4.1 Introduction;90 7.2;4.2 Experimental Approaches Adopted;94 7.3;4.3 DPI: Applications;95 7.4;4.4 Future Developments;106 7.5;4.5 Conclusions;108 7.6;Appendix 1 DPI: Background;108 7.7;Appendix 2 DPI: Theory;110 7.8;Appendix 3 DPI: Implementation;114 7.9;References;117 8;Total Internal Reflection Ellipsometry: Monitoring of Proteins on Thin Metal Films;120 8.1;5.1 Introduction;120 8.2;5.2 Total Internal Reflection Ellipsometry;121 8.3;5.3 Experimental Setup;125 8.4;5.4 Application Examples;128 8.5;5.5 Further Possibilities;132 8.6;References;133 9;Conformations of Proteins Adsorbed at Liquid- Solid Interfaces;134 9.1;6.1 Introduction;134 9.2;6.2 Experimental Techniques;140 9.3;6.3 Surface Effects on Both Protein Structure and Solvation by the ATR- FTIR Technique;145 9.4;6.4 Conclusion;157 9.5;References;157 10;Evaluation of Proteins on Bio-Devices;166 10.1;7.1 Introduction;166 10.2;7.2 Time-of-Flight Secondary Ion Mass Spectrometry ( TOF- SIMS);168 10.3;7.3 Analysis of Proteins on Bio-Devices;176 10.4;7.4 Summary;184 10.5;References;184 11;Fibronectin at Polymer Surfaces with Graduated Characteristics;190 11.1;8.1 Introduction;190 11.2;8.2 Gradated Substrate Physicochemistry;192 11.3;8.3 Fibronectin Exchange at a Constant Surface Concentration;196 11.4;8.4 Fibronectin Exchange at Variable Surface Concentrations;203 11.5;8.5 Relevance of the Interfacial Constraints of Fibronectin for Cell- Matrix Adhesion;210 11.6;References;212 12;Development of Chemical Microreactors by Enzyme Immobilization onto Textiles;214 12.1;9.1 Introduction;214 12.2;9.2 Nonconducting Cellulosic Textiles;216 12.3;9.3 Electron-Conducting Textile;242 12.4;References;257 13;Approaches to Protein Resistance on the Polyacrylonitrile- based Membrane Surface: an Overview;260 13.1;10.1 Introduction;260 13.2;10.2 Copolymerization Procedures;261 13.3;10.3 Poly(ethylene glycol) Tethering;267 13.4;10.4 Physical Adsorption;272 13.5;10.5 Biomacromolecule Immobilization;274 13.6;10.6 Biomimetic Modification;278 13.7;10.7 Conclusion;281 13.8;References;283 14;Modulation of the Adsorption and Activity of Protein/ Enzyme on the Polypropylene Microporous Membrane Surface by Surface Modification;286 14.1;11.1 Surface Modifications for Reducing Nonspecific Protein Adsorption;286 14.2;11.2 Surface-Modified PPMMs for Enzyme Immobilization;301 14.3;11.3 Conclusions;310 14.4;References;310 15;Nonbiofouling Surfaces Generated from Phosphorylcholine- Bearing Polymers;314 15.1;12.1 Introduction;314 15.2;12.2 Forces Involved in Protein Adsorption;315 15.3;12.3 Design of Phosphorylcholine-Bearing Surfaces;317 15.4;12.4 Mechanism of Resistance to Protein Adsorption on the MPC Polymer Surface;318 15.5;12.5 Fundamental Interactions Between MPC Polymers and Proteins;325 15.6;12.6 Recent Designs of Nonfouling Ph