Two-dimensional electrophoresis is the central methodology in proteome research, and the state of the art is described in detail in this text, together with extensive coverage of the detection methods available. Sufficient detail is given to allow the readers to apply these technologies to their own particular requirements.

Solubilization of proteins in 2D electrophoresis

2D electrophoresis with carrier ampholytes
2D electrophoresis with immobilized pH gradients
Detection of proteins on 2D electrophoresis gels
Blotting and immunoaffinity identification of 2-DE separated proteins
Identification of proteins by amino acid composition after acid hydrolysis
Identification by amino acid composition obtained from labeling
Identification of proteins by amino acid sequencing
Identification of proteins by mass spectrometry
Mass spectrometry of intact proteins from two-dimensional PAGE.