Proteomics in Practice
A Guide to Successful Experimental Design
Still the only concise practical guide to laboratory experiments in proteomics, this new edition now also covers DIGE technology and liquid-chromatography, while the troubleshooting section has been considerably extended.
Adopting a practical approach, the authors present the relevant techniques and explain the route to successful experimental design and optimal method selection. They cover such electrophoretic techniques as isoelectric focusing, SDS page, 2-D page, and DIGE, as well as liquid-chromatography techniques, such as ion exchange, affinity chromatography and reversed-phase HPLC. Mass-spectrometric techniques include MALDI, ESI, and FT ICR.
Generously illustrated, partly in color, the book also features updates of protocols as well as animations illustrating crucial methodological steps on a companion website.
Adopting a practical approach, the authors present the relevant techniques and explain the route to successful experimental design and optimal method selection. They cover such electrophoretic techniques as isoelectric focusing, SDS page, 2-D page, and DIGE, as well as liquid-chromatography techniques, such as ion exchange, affinity chromatography and reversed-phase HPLC. Mass-spectrometric techniques include MALDI, ESI, and FT ICR.
Generously illustrated, partly in color, the book also features updates of protocols as well as animations illustrating crucial methodological steps on a companion website.
Westermeier, Reiner
Naven, Tom
Höpker, Hans-Rudolf
ISBN | 9783527622306 |
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Artikelnummer | 9783527622306 |
Medientyp | E-Book - PDF |
Auflage | 2. Aufl. |
Copyrightjahr | 2008 |
Verlag | Wiley-Blackwell |
Umfang | 502 Seiten |
Sprache | Englisch |
Kopierschutz | Adobe DRM |