Thin-layer chromatography (TLC) is widely used particularly for pharmaceutical and food analysis. While there are a number of books on the qualitative identification of chemical substances by TLC, the unique focus here is on quantitative analysis. The authors describe all steps of the analytical procedure, beginning with the basics and equipment for quantitative TLC followed by sample pretreatment and sample application, development and staining, scanning, and finally statistical and chemometric data evaluation and validation. An important feature is the coverage of effect-directed biological detection methods. Chapters are organized in a modular fashion facilitating the easy location of information about individual procedural steps.

History of Planar Chromatography

The Theoretical Basis of Thin-Layer Chromatography (TLC)
The Stationary Phase in Thin Layer Chromatography (TLC)
The Mobile Phase in Adsorption and Partition Chromatography
Preparing and Applying Samples
Basis for TLC Development Techniques
Specific Staining Reactions
Bioeffective-linked Analysis in Modern HPTLC
Planar Chromatography Detectors
Diffuse Reflectance from TLC Layers
Fluorescence in TLC Layers
Chemometrics in HPTLC
Reciprocal Model
Statistics for Quantitative TLC
Planning an Analysis and Validation in TLC.