In the last few years the major effect that RNAi has had in invertebrate systems like C.elegans and drosophila is beginning to take hold in mammalian systems through both single gene knockdown experiments and genome-scale screens. In the next decade, there will no doubt be both notable successes and failures as we attempt to apply this genetic tool to various biological problems for the first time in academia and industry. Through the introduction of RNAi, mammalian systems have finally gained admittance to the pantheon of model genetic systems.

RNA Interference in Mammalian Cell Systems

RNAi Pathway in C. elegans: The Argonautes and Collaborators
Genetics and Biochemistry of RNAi in Drosophila
Role of Dicer in Posttranscriptional RNA Silencing
The Mechanism of RNase III Action: How Dicer Dices
MicroRNA Metabolism in Plants
Structure-Function Relationships Among RNA-Dependent RNA Polymerases
RNAi-Mediated Chromatin Silencing in Fission Yeast
A Role for RNAi in Heterochromatin Formation in Drosophila
RNA-Mediated Transcriptional Gene Silencing in Human Cells
RNA Silencing in Mammalian Oocytes and Early Embryos
Identifying Human MicroRNAs.