Rapid Cycle Real-Time PCR is a powerful analytical tool with broad application for the basic and applied life sciences. Compared with conventional PCR technology, Rapid Cycle Real-Time PCR is faster, has greater specificity, and is more easily adaptable for a variety of diagnostic tests, including qualitative, quantitative and mutation detection assays. This book provides general overviews of this technology for use in the clinical microbiology laboratory as well as specific diagnostic protocols for the detection of viral, bacterial and fungal pathogens and genetically modified organisms in human specimens and foodstuffs. All of these protocols have been developed, verified, and validated by experts in the field and should be of great interest for clinical microbiologists, pathologists, laboratory technologists as well as practicing physicians.

for Microbiology Volume of Rapid Cycle Real-Time PCR

Applications and Challenges of Real-Time PCR for the Clinical Microbiology Laboratory
I Bacteriology
Rapid Detection and Simultaneous Differentiation of Bordetella pertussis and Bordetella parapertussis in Clinical Specimens by LightCycler PCR
Rapid Detection and Simultaneous Differentiation of Legionella spp. and L. pneumophila in Potable Water Samples and Respiratory Specimens by LightCycler PCR
Homogeneous Assays for the Rapid PCR detection of Burkholderia pseudomallei 16s rRNA gene on a Real-Time Fluorometric Capillary Thermocycler
Rapid Detection of Toxigenic Corynebacterium diphtheriae by LightCycler PCR
Rapid Detection of Resistance Associated Mutations in Mycobacterium tuberculosis by LightCycler PCR
Duplex LightCycler PCR Assay for the Rapid Detection of Methicillin-Resistant Staphylococcus aureus and Simultaneous Species Confirmation
Rapid Detection of Group B Streptococci Using the LightCycler Instrument
Rapid Detection and Quantification of Chlamydia trachomatis in Clinical Specimens by LightCycler PCR
Quantification of Toxoplasma gondii in Amniotic Fluid by Rapid Cycle Real-Time PCR
Genospecies-Specific Melting Temperature of the recA PCR Product for the Detection of Borrelia burgdorferi sensu lato and Differentiation of Borrelia garinii from Borrelia afzelii and Borrelia burgdorferi sensu stricto
Rapid and Specific Detection of Coxiella burnetii by LightCycler PCR
Molecular Identification of Campylobacter coli and Campylobacter jejuni Using Automated DNA Extraction and Real-Time PCR with Species-Specific TaqMan Probes
Rapid and Specific Detection of Plesiomonas shigelloides Directly from Stool by LightCycler PCR
Quantification of Thermostable Direct Hemolysin-Producing Vibrio parahaemolyticus from Foods Assumed to Cause Food Poisoning Using the LightCycler Instrument
II Detection of Fungal Pathogens
Quantification and Speciation of Fungal DNA in Clinical Specimens Using the LightCycler Instrument
III Virology
Development, Implementation, and Optimization of LightCycler PCR Assays for Detection of Herpes Simplex Virus and Varicella-Zoster Virus from Clinical Specimens
Qualitative Detection of Herpes Simplex Virus DNA in the Routine Diagnostic Laboratory
Use of Real-Time PCR to Monitor Human Herpesvirus 6 (HHV-6) Reactivation
Simultaneous Identification of Five HCV Genotypes in a Single Reaction
Rapid Detection of West Nile Virus
Use of Rapid Cycle Real-Time PCR for the Detection of Rabies Virus
Development of One-Step RT-PCR for the Detection of an RNA Virus, Dengue, on the Capillary Real-Time Thermocycler
IV Detection of Genetically Modified Organisms (GMO)
Variation Analysis of Seven LightCycler Based Real-Time PCR Systems to Detect Genetically Modified Products (RRS, Bt176, Bt11, Mon810, T25, Lectin, Invertase).