This textbook covers the basic aspects of stem cell research and applications in regenerative medicine. Each chapter includes a didactic component and a practical section. The book offers readers insights into:

• How to identify the basic concepts of stem cell biology and the molecular regulation of pluripotency and stem cell development.
• How to produce induced pluripotent stem cells (iPSCs) and the basics of transfection.
• The biology of adult stem cells, with particular emphasis on mesenchymal stromal cells and hematopoietic stem cells, and the basic mechanisms that regulate them.
• How cancer stem cells arise and metastasize, and their properties.
• How to develop the skills needed to isolate, differentiate and characterize adult stem The clinical significance of stem cell research and the potential problems that need to be overcome. 
• Evaluating the use of stem cells for tissue engineering and therapies (the amniotic membrane)
• The applications of bio-nanotechnology in stem cell research.
• How epigenetic mechanisms, including various DNA modifications and histone dynamics, are involved in regulating the potentiality and differentiation of stem cells.
• The scientific methods, ethical considerations and implications of stem cell research.

Chapter 1. Basic Concepts of Stem Cells: The Facts and Hype in Stem Cells Research and Development

Chapter 2. Embryonic and Pluripotent Stem Cells
Chapter 3. Adult Stem Cells: Hematopoietic Stem Cells, and Control Of Hematopoiesis
Chapter 4. Adult Stem Cells: Adult Stem Cells: Mesenchymal Stromal Cells, Endothelial Progenitor Cells and Pericytes
Chapter 5. Cancer Stem Cells and the Development of Cancer
Chapter 6. Stem Cell Applications In Metabolic Disorders: Diabetes Mellitus
Chapter 7. Epigenetics in Stem Cell Biology
Chapter 8. Practicum: Isolation of Bone Marrow and Adipose Tissue Mesenchyme Stromal Cells
Chapter 9.  Induced Pluripotent Stem Cells: Biology, History and in-vitro Generation
Chapter 10. Tissue Engineering Modalities and Nanotechnology
Chapter 11.  Practicum: Scaffold engineering using the amniotic membrane
Chapter 12. The Scientific Method, Ethical Consideration and Implications in Stem Cell Research.