The validation and standardization of fluorescence methods is still in its infancy as compared to other prominent analytical and bioanalytical methods. Appropriate quality assurance standards are however a prerequisite for applications in highly regulated fields such as medical diagnostics, drug development, or food analysis.

For the first time, a team of recognized international experts has documented the present status of quality assurance in fluorescence measurements, and outlines concepts for establishing standards in this field.

This second of two volumes covers applications of fluorescence for bioanalysis and biomedical diagnostics.

Fluorescence Microscopy

Need for Standardization of Fluorescence Measurements from the Instrument Manufacturer's View
Characterization and Calibration in Wide Field and Sectioned Fluorescence Microscopy SIPcharts
Quantitative Fluorescence Microscopy: Considerations and Controls
Comparability of Fluorescence Microscopy Data and Need for Instrument Characterization of Spectral Scanning Microscopes
Fluorescence Lifetime Imaging Microscopy: Quality Assessment and Standards
Single Molecule Spectroscopy
State of the Art and Novel Trends in Fluorescence Correlation Spectroscopy
Single Molecule Spectroscopy: Instrumentation and Multiparameter Detection
Fluorescence-Based Microarray Technology: Applications, Future Trends, and Need for Standardization
DNA Microarrays: Applications, Future Trends, and the Need for Standardization
Comparability of Microarray Experiments from the Instrument and the Sample Site and Approaches Towards Standardization
Microarray Technology: Unresolved Issues and Future Challenges from a Regulatory Perspective
Protein Arrays and Fluorescence Detection: Applications and Limitations
Flow Cytometry
Flow Cytometry: Instrumentation, Applications, Future Trends and Limitations
Flow Cytometry Quality Assurance
Approaches to Quantitation in Flow Cytometry
Fluorescence Immunoassays
Immunoassays: Basic Concepts, Physical Chemistry and Validation
Time-Resolved Fluorometric Immunoassays; Instrumentation, Applications, Unresolved Issues and Future Trends
Particle-Based Assays: Applications and Unresolved Issues
Advances in Fluorescence Enzyme Detection Methods
Quantitative PCR
Quantitative Real-Time PCR: Fluorescent Probe Options and Issues
Fluorescence In Situ Hybridization and Immunohistochemistry
CellularBioimaging in Fluorescent Cancer Biomarker Evaluation: Validation, Technologies and Standards Development
Fluorescence Technologies in Biomedical Diagnostics
Fluorescence Techniques in Biomedical Diagnostics: Instrumentation, Analysis and Unresolved Issues
In-vivo Fluorescence Imaging: Applications, Future Trends & Approaches to Standardization.