This book, the second of two volumes on the Gentianaceae, is devoted to aspects of biotechnology and their applications. It consists of 18 chapters and covers micropropagation by means of organogenesis or somatic embryogenesis, and single cell manipulation of various species belonging to the horticultural genera Blakstonia, Centaurium, Gentiana, Gentianalla and Swertia. Furthermore, the application of somatic cell hybridization, haploidization and genetic variation arising from tissue and organ culture for the production of plants with new horticultural traits, such as new flower colors or sizes, or with special pharmaceutical values, is treated in detail. Also discussed are molecular markers that facilitate breeding and cultivar identification, the preservation of genetic resources by cryopreservation, the postharvest physiology of cut Gentian flowers and potted plants, and different analytical methods for the evaluation of Gentians as sources of secondary metabolites, such as xanthones and flavonoids, secoiridoids and C-glucoflavonoids, and their positive impacts on human health.

This volume as well as the companion book The Gentianaceae - Volume 1: Characterization and Ecology will serve as key reference works for scientists and students in the fields of botany, plant breeding, biotechnology and horticulture, as well as professional gardeners.

<p>Systems of plant regeneration in gentian <i>in vitro</i> cultures

<i>In vitro</i> manipulation and propagation of <i>Gentiana</i> L. species from the Ukrainian flora
<i>In vitro</i> studies and biotechnology of Taiwan native species of family Gentianaceae
Biotechnology and phytochemistry of <i>Gentianella</i> species from the central regions of the Balkan Peninsula
The role of arabinogalactan proteins in <i>Centaurium erythraea </i>Rafn morphogenesis <i>in vitro.-</i> Somatic embryogenesis in long-term cultures of <i>Gentiana lutea</i> L. in the presence of osmotic stress
Protoplast culture and somatic cell hybridization of gentians
Haploid and doubled haploid plants production in gentian (<i>Gentiana</i> spp.)
Genetic variation induced by tissue and organ culture in <i>Gentiana</i> species
Molecular breeding of Japanese gentians - Applications of genetic transformation, metabolome analyses, and genetic markers
Cryopreservation of Gentianaceae: trends and applications
Postharvest physiology of flowers<i> </i>from family Gentianaceae
Tissue and organ cultures of gentians as potential sources of xanthones and flavonoids
Bioactive secondary metabolites in several genera of the Gentianaceae species from the central region of Balkan Peninsula
Profiling, isolation, chemical characterisation and distribution of Gentianaceae constituents
Phytochemistry and Biotechnological Approches
<i>Gentianae</i><i> </i><i>radix</i>.-<i> </i>Gentians used in South America as antimalarial agents .</p>