1;Preface;62;Contents;83;Contributors;104;Two-Photon Imaging of the Immune System: A Custom Technology Platform for High-Speed, Multicolor Tissue Imaging of Immune Responses;144.1;1 Introduction;154.2;2 Description of Custom Two-Photon Instrumentation;244.3;3 Representative Data;344.4;4 Future Perspectives;364.5;References;395;Visualizing Intermolecular Interactions in T Cells;435.1;1 Introduction;445.2;2 Molecular Recruitment to the Immunological Synapse;455.3;3 Molecular Interactions in the Immunological Synapse;495.4;4 Variability in the Form of the T Cell:APC Interface;535.5;5 Concluding Remarks;555.6;References;556;Multiscale Analysis of T Cell Activation: Correlating In Vitro and In Vivo analysis of the Immunological Synapse;596.1;1 Introduction;606.2;2 Parameters in T Cell Activation;616.3;3 Immunological Synapses;666.4;4 Coordination of Antigen Recognition and Migration;716.5;5 Conclusions;766.6;References;777;T Cell Migration Dynamics Within Lymph Nodes During Steady State: An Overview of Extracellular and Intracellular Factors Influencing the Basal Intranodal T Cell Motility;837.1;1 Introduction;857.2;2 Extracellular Factors Influencing the Intranodal Migration Behavior of T Lymphocytes;887.3;3 Intracellular Factors Contributing to Intranodal T Cell Motility;1017.4;4 Computational Simulation of T Cell Migration and Interaction Dynamics: The In Silico Lymph Node Model;1067.5;5 Conclusion;1107.6;References;1128;Chemoattractant Receptor Signaling and Its Role in Lymphocyte Motility and Trafficking;1188.1;1 Introduction;1198.2;2 Major Chemoattractant Receptors that Function in Lymph Node Homing, Motility, and Egress;1198.3;3 Major Mediators of Chemoattractant Receptor Signaling;1218.4;4 Imaging Lymphocyte Trafficking and Motility;1248.5;5 Conclusions;1348.6;References;1359;New Insights Into Leukocyte Recruitment by Intravital Microscopy;1399.1;1 Introduction;1409.2;2 Advantages and Disadvantages of IVM;1419.3;3 Capturing;1429.4;4 Rolling;1459.5;5 Arrest;1479.6;6 Postadhesion Events;1499.7;7 Transmigration;1509.8;8 Organ Specific Recruitment;1529.9;9 Perspectives;1539.10;References;15410;Visualizing the Molecular and Cellular Events Underlying the Initiation of B-Cell Activation;16310.1;1 Introduction;16410.2;2 Tools for Visualizing B Cell Activation Events;16510.3;3 Visualizing Molecular Events During the Initiation of B Cell Activation;16810.4;4 Visualizing Intercellular Events During the Initiation of B Cell Activation In Vivo;17410.5;5 Conclusions;18110.6;References;18211;Tracking the Dynamics of Salmonella Specific T Cell Responses;18811.1;1 Introduction;18911.2;2 Studying Immunity to Salmonella;18911.3;3 Visualizing Salmonella In Vivo;19111.4;4 Adaptive Immunity in the Murine Typhoid Model;19111.5;5 Tracking Antigen-Specific Responses in Salmonella Infection;19311.6;6 Development and Function of Th1 Cells During Salmonella Infection;19911.7;7 Conclusion;20111.8;References;20112;Imaging Listeria monocytogenes Infection In Vivo;20812.1;1 Introduction;20912.2;2 Listeria Infection in Mice as a Model of Host Pathogen Interactions;21012.3;3 Innate Immune Responses to Listeria;21012.4;4 Adaptive Immunity to Listeria;21112.5;5 In Vivo Bioluminescence Imaging Studies of Listeria Infection;21212.6;6 In Vivo Fluorescence Imaging Techniques;21412.7;7 Confocal Laser Scanning Microscopy;21712.8;8 Application of CLSM in Studying Listeria Pathogenesis;21812.9;9 Introduction to Two-Photon Microscopy;21812.10;10 2P Microscope Design;22012.11;11 Live Tissue Imaging and In Vivo Cell Dynamics;22212.12;12 2P Imaging Data Analysis;22312.13;13 2P Microscopy of Listeria Infection;22412.14;14 Using 2P Microscopy to Assess the Presentation of Bacterial Antigen In Situ;22512.15;15 Single-Cell Tracking of MZ Macrophages and PALS DCs;22612.16;16 2P Microscopy can Detect Individual Bacteria in Infected Host Cells;22712.17;17 Future Directions;22912.18;References;229