Researchers have used a variety of techniques over the past century to gain fun- mental insights in the field of immunology and, as technology has advanced, so too has the ability of researchers to delve deeper into the biological mechanics of immunity. The immune system is exceedingly complex and must patrol the entire body to protect us from foreign invaders. This requires the immune system to be highly mobile and adaptable - able to respond to diverse microbial challenges while maintaining the ability to distinguish self from a foreign invader. This latter feature is of great importance because the immune system is equipped with toxic mediators, and a failure in self/non-self discrimination can result in serious diseases. Fortunately, in most cases, the immune system operates within the framework of its elegant design and protects us from diverse microbial challenges without initiating disease. Because the immune system is not confined to a single tissue, a comprehensive understanding of immunity requires that research be conducted at the molecular, cellular, and systems level. Immune cells often find customized solutions to h- dling microbial insults that depend on the tissue(s) in which the pathogen is found.

Two-Photon Imaging of the Immune System: A Custom Technology Platform for High-Speed, Multicolor Tissue Imaging of Immune Responses

Visualizing Intermolecular Interactions in T Cells
Multiscale analysis of T cell activation: correlating in vitro and in vivo analysis of the immunological synapse
T Cell Migration Dynamics Within Lymph Nodes During Steady State: An Overview of Extracellular and Intracellular Factors Influencing the Basal Intranodal T Cell Motility
Chemoattract Receptor Signaling and Its Role in Lymphocyte Motility and Trafficking
New Insights Into Leukocyte Recruitment by Intravital Microscopy
Visualizing the Molecular and Cellular Events Underlying the Initiation of B-Cell Activation
Tracking the Dynamics of Salmonella Specific T Cell Responses
Imaging Listeria monocytogenes Infection In Vivo
Inflammation on the Mind: Visualizing Immunity in the Central Nervous System
Multiphoton Imaging of Cytotoxic T Lymphocyte-Mediated Antitumor Immune Responses.